Unformatted text preview: column. Continue to add until beads settle. Use to 5 times volume of 50 mM TrisCl pH8 to equilibrate the column. After volume passes through the resin close the small yellow lid. Level of buffer should be just above the resin bed. Read the absorbance value of blue dextran, using 50mM TrisCl buffer as a blank. Use 3 column volume of 20% entonlol to wash the column. Then leave 10 mL above the bed, cap the column, close the outlet, gently invert the column to generate slurry and pour into container. Rinse empty column with water the D water and return it....
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- Spring '11
- Glass, Column, High performance liquid chromatography