Gel-Filtration Chromatography

Gel-Filtration Chromatography - Gel-Filtration...

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Gel-Filtration Chromatography Aim and Importance: Learn to properly pack a gel-filtration column and separate a mixture of three colored compounds on the basis of differences in molecular weight. Chromatography is a basic and very important skill that all chemists must know about in order to easily separate and/or purify mixtures. Gel filtration Chromatography is a technique that takes advantage of size variations of molecules in mixtures. Background: Chromatography is the collective term for a set of laboratory techniques for the separation of mixtures different components are flushed through the system at different rates. There are many types of chromatography, but all involve dissolving the compounds to be separated in a liquid solution. Gel filtration involves the separation of molecules of different dimensions based on the size as they pass through a gel medium packed in a column. Molecules move through a bed of permeable beads that have a range of pore sizes that determine the size of molecules that can be separated. Smaller
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This note was uploaded on 10/25/2011 for the course ALL 101 taught by Professor All during the Spring '11 term at Cameron University.

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Gel-Filtration Chromatography - Gel-Filtration...

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