Solution making - Solution making Aim and Importance: In...

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Solution making Aim and Importance: In experimental biochemistry buffers are extremely important because virtually all biochemical studies are carried out in some type of buffer, making the knowledge of solution and buffer creation essential. Also most of the time concentrated stock solutions are kept in laboratories and the experimenter has to create the dilute solution required for the buffer needed. Therefore every student needs to learn the process and calculations required in solution and buffer making. Background: Most simply, a buffer functions to resist changes in hydrogen ion concentration as a result of internal and environmental factors. Buffers usually contain an acid and one of its salts or a base and one of its salts. One of the two compounds in a buffering system acts as a proton donor, releasing H + when pH rises, and the other as a proton acceptor, taking up H + when pH drops. Because enzymes are so pH-sensitive, not only for their activity but also for their stability in solution, buffers are very important. In a lab, researchers keep all of their proteins in buffered solutions. Therefore, beginning
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This note was uploaded on 10/25/2011 for the course ALL 101 taught by Professor All during the Spring '11 term at Cameron University.

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Solution making - Solution making Aim and Importance: In...

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