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Biology 141 - Week 9 slides - Fall 2011 - GM

Biology 141 - Week 9 slides - Fall 2011 - GM - Yeast...

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Yeast: Replica-plating and complementation L. Mclane and A. Escobar
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Review of definitions Chromosomes Genes Alleles - Inherited genetic material - Prokaryotes vs. Eukaryotes - Smallest unit of heritable material - Blueprint of a protein or functional RNA molecule - Alternative forms of genes L. Mclane and A. Escobar
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Complementation: the basics Cell X Cell Y Haploid (n) Normal gene A Haploid (n) Mutant gene a X Can Cell X survive after it has been mated with Cell Y? Why or why not? L. Mclane and A. Escobar
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Complementation in yeast A B C Enzyme Z Enzyme Q a x α Z+ Q - Z- Q + Z+Q- Z-Q+ Gene Z Gene Q Metabolic Intermediates L. Mclane and A. Escobar
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Z+ Q- Metabolic Intermediates A B Enzyme Z Gene  Z mutant Gene  q C Enzyme Q A Enzyme Z Enzyme Q mutant Gene  z Gene  Q B Z- Q+ Metabolic Intermediates C Z+ Q- Z- Q+ A B C Enzyme Z Enzyme Q Gene  Z Gene  Q Metabolic Intermediates α a Z+Q+ A B C Enzyme Z Enzyme Q Gene  Z Gene  Q Metabolic Intermediates a or  α L. Mclane and A. Escobar X X
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Useful Auxotrophic Markers Some strains are prototrophic (wild type) Many laboratory strains are auxotrophic for one nutrient or another - dependent on another source for a nutrient URA3 LEU2 HIS3 TRP1 ADE2 ADE3 LYS2 others L. Mclane and A. Escobar
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Genetic complementation of auxotrophic markers a x α TRP1 trp1 Will not grow without TRP supplemented, so it is Trp- TRP1 trp1 Will grow without TRP supplemented, so it is Trp+ L. Mclane and A. Escobar
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L. Mclane and A. Escobar
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L. Mclane and A. Escobar
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