C om pr eme d41 1c om 15 which of the following terms

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Unformatted text preview: tose operon in E. coli? A. inducible system B. repressible system C. negative control D. positive control E. constitutive Answer: D - PR EME D41 1.C OM - PR EME D41 1.C OM The table below lists several genotypes associated with the lac operon in E. coli. To answer each of the following four questions, indicate with a “YES” or a “NO“ whether _-galactosidase would be expected to be produced at induced levels. = wild type repressor I+ = mutant repressor (unable to bind to the operator) I= mutant repressor (insensitive to lactose) Is = wild type operator O+ c = constitutive operator (insensitive to repressor) O _ -galactosidase production Genotype No Lactose ; On Lactose I + O+ Z+/ F’ I + O+ Z+ _____?____; ____?___ - PR EME D4 11. COM 16. - PR EME D4 11. COM A. NO ; YES B. NO ; NO C. YES ; NO D. YES ; YES Answer: A 11. _ -galactosidase production No Lactose ; On Lactose I – Oc Z-/ F’ I – Oc Z- _____?_____; ____?___ PRE MED 411 .CO MPRE M ED4 17. COM Genotype A. NO ; YES B. NO ; NO M .CO 411 MED - PR E - PR EME D41 1.C OM C. YES ; NO D. YES ; YES Answer: B _ -galactosidase production No Lactose ; On Lactose D41 1.C OM Genotype - PR EME I – Oc Z+/ F’ I – O+Z+ 18. D41 1.C OM - PR EME D41 1.C OM A. NO ; YES B. NO ; NO C. YES ; NO D. YES ; YES Answer: D _____?_____; ___?____ - PR EME Genotype __________; _______ 1.C OM I s Oc Z-/ F’ I s O+ Z+ 19. _ -galactosidase production No Lactose ; On Lactose - PR EME D41 1.C OM - PR EME D41 A. NO ; YES B. NO ; NO C. YES ; NO D. YES ; YES Answer: B Structural gene Transcription COM - PR EME D41 1.C OM - PR EME D41 1.C OM 20. Given the diagram below, what type of control, positive or negative, is operating? - PR EME D4 11. Regulatory protein 11. COM A. Negative B. Positive Answer: B PRE MED 411 .CO MPRE M ED4 11. COM - PR EME D4 21. Some vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region. What term is given to this advantageous arrangement of...
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This document was uploaded on 10/27/2011 for the course BIL 250 at University of Miami.

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