Some vectors such as puc18 and others of the puc

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Unformatted text preview: restriction sites? A. palindrome B. consensus sequence C. polylinker D. _ -galactosidase E. complementation M .CO 411 MED - PR E 1.C OM Answer: C OM - PR EME D41 1.C OM - PR EME D41 22. In the context of molecular genetics, reverse translation refers to A. assembling a DNA sequence from an amino acid sequence. B. assembling a RNA sequence from a DNA sequence. C. translating in the 3' to 5' direction. D. transcribing first, then translating. E. making an amino acid sequence from a DNA sequence. Answer: A D41 1.C OM - PR EME D41 1.C 23. Below are four processes common to most cloning experiments: transforming bacteria (1) plating bacteria on selective medium (2) cutting DNA with restriction endonucleases (3) ligating DNA fragments (4) D41 1.C OM - PR EME D41 1.C OM - PR EME Place components of the above list in the order in which they would most likely occur during a cloning experiment. A. 1 >> 2 >> 3 >> 4. B. 2>> 3 >> 1 >> 4. C. 4 >> 3 >> 2 >> 1. D. 3 >> 4 >> 1 >> 2. E. 3 >> 1 >> 4 >> 2. Answer: D - PR EME D41 1.C OM - PR EME D41 1.C OM - PR EME 24. Compared with prokaryotic chromosomes, eukaryotic chromosomes are A. large, mainly organized in monocistronic transcription units without introns. B. small, mainly organized in monocistronic transcription units with introns. C. large, mainly organized in polycistronic transcription units without introns. D. small, mainly organized in polycistronic transcription units without introns. E. large, linear, less densely packed with protein-coding genes, mainly organized in monocistronic units with introns. Answer: E - PR EME D4 11. COM - PR EME D4 11. COM 25. RFLPs and minisatellites are commonly used in recombinant DNA technology to A. cleave DNA of interest. B. generate pharmaceutical products of interest. C. serve as recombinant DNA vectors. D. map genes and DNA fingerprints. E. substitute for oligonucleotides. Answer: D COM 26. Polyploid indiv...
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