BISC12labreport-Daphnia temp

BISC12labreport-Daphnia temp - Yontii Wheeler...

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Yontii Wheeler [email protected] Lab Partners: Ami, Harleen, Marissa, Megan, and Paola Biology 12 Spring Semester Date Collected: January 27, 2010 Date Due: February 3, 2010 Professor Doebel GWID:G49559369 The Affect of Temperature on Daphnia 1
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Introduction: Purpose: The purpose of this experiment was to determine whether changing the temperature of the water Daphnia swim in would affect their movement and behavior through observation and statistical analysis. In this lab, before beginning to the experiment, this biologist and her lab partners found out the optimal pH and temperature for Daphnia. Afterwards, we examined Daphnia in their optimal temperature and pH and determined their behavior. We determined that, at their optimal temperature of around 20 degrees C, Daphnia swim in different directions but after swimming in a certain direction for about 3 seconds, they stop swimming and float towards the bottom for 2 seconds. Afterwards, they repeat this process in another direction. The Daphnia also tend to bounce a little when they swim in their optimal temperature water. With these known facts, we were able to construct an experiment to test their behavior in different temperatures of water. Null Hypothesis : There is no relationship between the movement and behavior of Daphnia, and temperature of their environment. Alternative Hypothesis : If Daphnia are placed in different temperatures, then their behavior and movement will be affected. 2
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Materials : 5 thermometers, 3 hot plates, a bucket of ice, 5 250 ml beakers, 5 test tubes with 5 Daphnia in each, 5 timers, 2 beaker tongs, 5 test tube clamps, deionized water, black marker, and a test tube rack Methods : 1. Obtain materials. 2. Fill a beaker with 250 ml of deionized water at 20 degrees Celsius. This is about room temperature but if water is not already this temperature, take appropriate steps (heat or cool it) in order for it to reach this temperature. 3. Place a beaker of 250 ml of deionized water on a hot plate at 60 degrees Celsius with a thermometer inside. 4. Watch the temperature of the water in that beaker (using the thermometer) and take it off the hot plate when it reaches 40 degrees C. 5. Repeat steps 3 and 4 with another 250 ml beaker but take beaker off hot plate when the water reaches 30 degrees C. 6. Fill 2 other 250 ml beakers with deionized water and place them on an ample amount of ice. 7. Put a thermometer in each beaker. 8. Take one beaker off ice when it is 10 degrees C. 9. Take the other beaker off the ice when it is 0 degrees C. 10. Place all beakers on tables with thermometers inside. 11. Get the 5 test tubes containing Daphnia and place them in each of the 5 beakers with thermometers inside. 12. Have all lab members take note of the behavior of the Daphnia under each of the 5 different temperature environments.
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