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Sea Urchin Lab in Ethanol

Sea Urchin Lab in Ethanol - Yontii Wheeler...

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Yontii Wheeler [email protected] Lab Partners: Stephanie Mander, Anna Benson, and Nadirah Waites BISC O11 Lab Section that met 6:00-8:50 on Thursdays Fall Semester Data Collected: November 19, 20, and 21 2009 Date Report Written: December 9, 2009 Date Due: December 10, 2009 by 5:00 pm Daniela Andriamalala Thursday November 30, 2009 GWID: G49559369 The Effect of Ethanol on Lytechinus Pictus Embryo Development Abstract :
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In this lab, this biologist studied the effect of ethanol on lytechinus pictus embryo development. There was a control of lytechinus pictus embryo in a petri dish filled with distilled water being compared to an experimental petri dish with lytechinus pictus embryos in a medium of ethanol. When comparing the two dishes, data was collected by counting the number of embryo in each stage of lytechinus pictus development. After creating solutions of egg and sperm, fertilization, and placing the fertilized eggs into the control dish and the experimental dish, this scientist counted the number of embryo in each stage of development once every 24 hours after the above was performed. Then, the data (number of embryo from each petri dish, the control and the experimental dish) was compared and a chi square value was found. This scientists found that the chi square value for Friday was 0.4136 and less than the tabulated value so the alternative hypothesis was rejected and the null hypothesis accepted. This is because there was not enough time for the ethanol to effect the embryo development. But after one more day, on Saturday, the chi square value was found to be greater than the tabulated value and it was found that the ethanol did effect embryo development.
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Introduction : Lytechinus Pictuss are a part of the echinoderm family. The lytechinus pictuss used in this experiment, lytechinus pictus are from a wild type in Florida. It was necessary to stimulate the lytechinus pictuss in order to receive egg and sperm for them. The egg was fertilized data was collected in regards to the the embryos development in different chemicals. Most would ask why this scientist chose to study lytechinus pictuss. Lytechinus Pictuss were used because their embryo is specific to certain toxins, the timing of the stages of fertilization development doesn’t vary, and time for fertilization is short. Therefore, it is best to use lytechinus pictus to study the effects of toxins on its embryo because the factors stated above make it easy to use in the lab and manipulate in order to fit the criteria for the experiment. In this lab, the “chemical”, ethanol was used to study its effect(s) on lytechinus pictus embryo development. The ethanol mimicked a toxin in the ocean. The purpose of this lab was to determine if there was an effect of ethanol on the maturation of the embryo of lytechinus pictus. If there was a noticeable affect, then it was determined in what stage the embryo’s development was halted or stalled due to the ethanol.
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