Biological Transport Lab Report

Biological Transport Lab Report - Biological Transport Lab...

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Biological Transport Lab Lab Partners: Anna and Nadirah Due: Thursday September 24, 2009 Biological Transport Lab Yontii Wheeler Group: Bio Busters Statement: Osmosis is the diffusion of water down it’s concentration gradient through a semipermeable membrane. What is the quantitative relationship between temperature and the rate of osmosis of a molecule? Hypothesis: As the temperature of a solution is increased, the rate of osmosis will also increase. Null Hypothesis: As the temperature of a solution s increased, the rate of os- mosis is not affected. Procedure: 1. Each person that will be involved in this lab experiment must first, put on two latex gloves. 2. Using a micropipette, put 1 ml of .005 M of Crystal Violet Dye into each of test tubes. Be careful and try to keep the dye in the test tube. 3. Label each test tube. One test tube with the letter ‘R’ and the other with the number ‘60’ using a sharpie. 4. With a graduated cylinder, measure out 9 ml of deionized water and pour it into test tube ‘R’. 5. Repeat step 3 and pour it into the test tube labeled ‘60’. 6. Gently stir the mixture in the test tube then place a small piece of parafilm around the edges of the top of the test tubes to avoid evaporation and ensure that the liquid in the solution is not contaminated. 7. Fill a 250 ml beaker 3/4 of the way full with deionized water. 8. Place the beaker in the incubator for 15 minutes. 9. While the beaker is in the incubator, fill another 20 ml beaker 3/4 of the way, with deionized water and set it aside. 10. With the help of another lab partner, carefully pour the contents the test tube labeled ‘R’ into one of the dialysis bags. Be careful not to touch the middle of the dialysis bag; the oil from your hands could transfer to the pores in the bag and clog them, deterring osmosis. 11. Using a micropipette, put, 400 micro liters of the test tube contents ‘R’ to a cuvette. 12. Add 400 micro liters of the test tube contents ‘60’ to another cuvette. Make sure to keep the two cuvettes separate and to know which test tube is which. 13.
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Biological Transport Lab Report - Biological Transport Lab...

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