Protein Lab Tables

Protein Lab Tables - .76 nm 3 6.0 mg/ml 0.5 ml 1.5 ml.64 nm...

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Page 16: Table 1: Test Tube H2O (ml) 10 mg/ml BSA Stock Solu- tion (ml) Total Volume (ml) Final Protein Concentration (mg/ml) 1 0 ml 1.25 ml 1.25 ml 10 mg/ml 2 .25 ml 1 ml 1.25 ml 8.0 mg/ml 3 0.5 ml .75 ml 1.25 ml 6.0 mg/ml 4 .75 ml .5 ml 1.25 ml 4.0 mg/ml 5 1 ml .25 ml 1.25 ml 2.0 mg/ml Table 2: Test Tube Protein Concen- tration (mg/ml) Protein (ml) 0.5 M KCL Biuret: Reagent (ml) Biuret: Abs at 540 nm Blank (7) 0.0 mg/ml 0 0.5 ml 1.5 ml .13 nm 1 10.0 mg/ml 0.5 ml 0 1.5 ml .88 nm 2 8.0 mg/ml 0.5 ml
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Unformatted text preview: .76 nm 3 6.0 mg/ml 0.5 ml 1.5 ml .64 nm 4 4.0 mg/ml 0.55 ml 1.5 ml .47 nm 5 2.0 mg/ml 0.5 ml 1.5 ml .30 nm Unknown (6) 1:4 dilution 0.5 ml 1.5 ml .67 nm Page 17: Blood serum from which species: Sheep o Serum protein dilutions: 1:4 (mg/ml) 1:8 o Serum protein concentrations: .670 mg/ml .449 mg.ml Page 18 Protein Standards Distance Migrated 250 150 100 75 50 37 25 20...
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