Protein Lab Tables

Protein Lab Tables - .76 nm 3 6.0 mg/ml 0.5 ml 1.5 ml.64 nm...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Page 16: Table 1: Test Tube H2O (ml) 10 mg/ml BSA Stock Solu- tion (ml) Total Volume (ml) Final Protein Concentration (mg/ml) 1 0 ml 1.25 ml 1.25 ml 10 mg/ml 2 .25 ml 1 ml 1.25 ml 8.0 mg/ml 3 0.5 ml .75 ml 1.25 ml 6.0 mg/ml 4 .75 ml .5 ml 1.25 ml 4.0 mg/ml 5 1 ml .25 ml 1.25 ml 2.0 mg/ml Table 2: Test Tube Protein Concen- tration (mg/ml) Protein (ml) 0.5 M KCL Biuret: Reagent (ml) Biuret: Abs at 540 nm Blank (7) 0.0 mg/ml 0 0.5 ml 1.5 ml .13 nm 1 10.0 mg/ml 0.5 ml 0 1.5 ml .88 nm 2 8.0 mg/ml 0.5 ml 0 1.5 ml
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Background image of page 2
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: .76 nm 3 6.0 mg/ml 0.5 ml 1.5 ml .64 nm 4 4.0 mg/ml 0.55 ml 1.5 ml .47 nm 5 2.0 mg/ml 0.5 ml 1.5 ml .30 nm Unknown (6) 1:4 dilution 0.5 ml 1.5 ml .67 nm Page 17: Blood serum from which species: Sheep o Serum protein dilutions: 1:4 (mg/ml) 1:8 o Serum protein concentrations: .670 mg/ml .449 mg.ml Page 18 Protein Standards Distance Migrated 250 150 100 75 50 37 25 20...
View Full Document

This document was uploaded on 10/27/2011 for the course BIO 10002 at GWU.

Page1 / 2

Protein Lab Tables - .76 nm 3 6.0 mg/ml 0.5 ml 1.5 ml.64 nm...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online