Exam 3 & Final Spring 2009

Exam 3 & Final Spring 2009 - COM 11. ED4 ^ -i^. .\\...

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Unformatted text preview: COM 11. ED4 ^ -i^. .\\ FORM' i) 2 Exam*3 & Final May 07, 2oo9 COM - PR EM P Bil 255Springzoog Luis Glaser& CharlesMallen ED4 11. Directions: From arrrong choices eachquestion, pIcK the BEST choice. (2oo points) the for COM The G proteinconsists subunits: p, y. lVhichstatement correc.t./ of3 a, rs a' only the a subunitis activein signaltransdrrction b. The o,and py subunitsare activein srgnal transduction c. Signaltransduction be both stimulaton.as can r,r.ell inhibitorr. as d . a a n dc a p p l v oqb nndcapph. - PR EM ED4 11. t. - PR EM @ 11. COM - PR EM Secreted proteins are? (Think r.crycarefulll) a S-r'nthesizedthe cltuDlasm in and then assembled vesicles into that deliverthem to the cytoplasmic membrane b. Cutranshtionllll inieried into rhe lumenof the ER c. Trarerse Golginetnork on the,.i\.a] the crloplasrrr the to d- a and l are correct b urrdc arc correct \ - PR EM s a. L#s ofp53 gene @,iand b appry ED4 1) ED4 11. COM z. whjch ofthe followingevents rikelyto predispose cellto become cancer are a a celr? b. Lossof DNA repairprotein c. Decreaie cilurar ATp rer.els a. o una . uppr.,' in COM 11. ED4 Lr .Asignalanchorsystem? T. I-s alr|e]s at the anrinoterminusof a protcin b. Is alu'aysat the carboxy* terminus of a protein c. cDntranslationally inseftsa proteininto the ER membr.ane the form ofan u-helix in COM - PR EM ';i"' "::ffiil,,:1""ft,51"li: u' ,:,"":';Jffff,:1"::ff:l'l'llHf:,illH i:,T.x'":..4:?T:l;::,::i;ff - PR EM ED4 11. 6. Proteins that failto fold r)roperly the ER are? in a. Degraded proteases the Fr,R by in Returnedto the cltoplasmand degraded lysosomes in ^b. ' a\Returned to the otoplasm and defracledby proteosomes - PR EM ED4 11. COM 7. A protein that doesnot fold properl; in the ER could recl clc permanentlybetrveen glucosylatecl a 'a.AslorvpioteaseinthiDi--'-fbrm and a glucose c],cle prevcntcd is br.? .freeform. This permanent b. A slo$ manosidase thc ER in c. Calnexin getstired and refuses plav to MPRE MED 411 .CO V MPRE M ED4 11. COM 8 Proteins destined the lysosome proteinsdestined secretion to and for both containN-linkedcomplcxcarbohydrates. t - T h eu L u r a t ed e c i s i r n h a l d e r e r m i n r ns c r ea p r o t e i n ot. q s e f l e r . r s i ( r h i n k . i r r e f r l h . ) : .h g- r. " h es e t l u e n c e t h e c a r b o h l d r a t e of t.h"."qu"n..,"ftn"-prli"l,i 7a.'l ^ tumor cells the Rasprotein frequently?, a. Shor.r.s enhancecl GTpaseacti\io. ilb\ LacksGTpaseactivity { ro Glugacon stimulates release the ofglucose (livercells)? Readthe choices from hepatocltes caretrrlly: a.This is dueto the interaction ofGlucagon uith g)l cogen phosphoniase b. Rcquires increase the lerelsof cAMp in tlie hepatocl-tes an in c.. the resultof phospho4latjon Is ofphc,,genphosphoryiase rl. r and c are correct i b and c'a.ec.,.r",.1 MED MPRE MED 411 .CO MPRE MED 411 .CO rz The bindingofACTH to its receptor the adrenal in cofte.r results a largeincrease intracellular in in cAMp. Each ACTH.rnoleculegenerates manv cAMp moleculesbecause? (think very:carefullj) a Eachreceptorcan acti\ ate ntanl c proteins Each_ protein G u.tiiri'" rnunyadenyl-cyclase molecules c.Adenyl-cyclase high catrhlic actirih has "p. and c apply "un t u,ra. ujrp1. $ ". r3 Upon addition of epidermalgronth factor (EGF) to cells,the EGF receptoris phosphorylatecl at multiple sites on ty'rosine residues. This phosphorylation? u l s i r r e l e v a n t s i g n a t r a n s d ^ r r c t bo n G F to l i yE f o i - p . P r o r i d e s i t e s o r a c l i \ a t i o n f o t h e rp r o t e i n sn t h ec e l l r. E;rch is specific a difft.rentpathwar. (d1b and c appii site for PRE {b v 411 .CO tr' Tunlor cells(cancer cells)inasolid tumor are likely to differfrom the normalcells-'' the tissue in -"]au""n,," oforigin? a. Activeslnthesisofanti8enicproteins p53 p.oi"in----oftelomerase [Lack of functionut d. u. b and c apptl A)a and b appl1. COM P a g e2 11. P - Test &Final Bil255s09 #3 - PR EM ED4 Form2 ED4 11. COM i 14. Apoptosisin cells resultsin? (Pick the bestanswer) a. an increasein the acti\"itvof NA'/K. ATPase b. actlvahon ot'a cas('ade proleolitic en4 mes (caspascs) ;qf . _ tc. frequently requiresthe releaseofcytochrome c from mitochryrdria QJb and c apply - PR EM WhenATP levelincrease the p cellsof the pancreas, in thc1, closethe K. channel. You expcctthe membrane potential to go from? ainterior negativeto positive b. interior positiveto negative COM 1b. ED4 11. COM - PR EM r5. To determineu'hethera chemicalis mutagenicyou first perform the Ames tcst, to do so !'ou require? yeastmutantsin B Galactosidase a. a setof different 11. setofdifferentSalmonella a mutantsin histidinebioslnthesis ra. b und c appll c. a lal liver erlr;rct e.aandcappll' The graphillustrates dependence the ofthe rateofglucose entr.r'into celldepenclent rvhether expresses a on it the filnctional differentproteinGlu-1or Glu-2.The normalconcentration ofglucose aboutS mM, you expect p is the cellsof the pancreaFtq havea transportmolecule like? ' -- 500 .b'lGlu a. Glu r z c. Either rvill be compatiblewith the tunction of the p cells a proteinis'l r8. The letinoblastoma a. An onco€lene b. A negativeregulatorof cell growth (a tumor suptrrressor) a . c. The proteinis inactivated phosphon'lation / by 6 d.rundhepplt E 11. COM - PR EM ED4 11. COM - PR EM ED4 11. 17. ED4 Guanacappli - PR EM 2 4 3 5 6 7 8 9 10 11 12 13 14 COM - PR EM ED4 11. COM E r l e r n s r o . c d n r ' a r i o n t g l L , c o s l€ M ) o m c is tg. Calnexin a cbaperone that assists qualitvcontrol in in the ER. It doesso bv? a. Bindingto mannose residues proteins in destined be secreted go to lysosomes to or b. Bindingto glucose residues suchproteins in c. The presence the glucose of residr.res indicates that the proteinis not properlyfolded d. b and c appll G)a and c appll - PR EM ED4 11. zo. 1\ hich ofthc follorringstatemcnts is/arecorrect? a. CellscontainoneCdK and mant'ct clin-s b. Cells containmanl CdK'sand oneclclin c. CellscontainmultipleCdK'sand multiplecvclins that pair at random .dr Cells pairs containmultipleCdK'sand multiplecyclins that form specific Malle+ - Cumulative 11. COM La!:-!rs&sso! MPRE M ED4 zr. The molecule depictcd the figureto the right is l? in "@ a) tatq acid b) sugar dipepticli d) nLrcleotide this e) choose answerif noneofiiese is correct cl) ionic bond HOR \ N H/ (. '€j lll C -C-N-C I -C z t tlr\ IiHH none of theseis correct MED 411 .CO zz. The tlpe of bond depicted the shadetl in areaabore is a? a) glltosidic bond b) hydrogenbond c) nLrcleotide bond ir 411 .CO MPRE 23. A molecular r'hich hasthe abilitvto makea copl ofitselfand directotherrrolecules replicate complex, to is to a) a mitoticspindle b) an abioticcatall-st -themselves, comrnonllreferred as? d) a coacen'ate e) choosethis ansrverif none of theseis correct \ i\a replicase MPRE MED 24. The thermophiiic bacteria that live in placcs deepdrr'elling as hltlrothernralrents chssifiedas? ale a) Eubacteria b) metaphytian cells c) true nuclear contiiningcells $ archaeabrcteria e) noneofthese .CO MPRE ::6. Therhemicalfunctionalgroup [C=O]can bestdefineby which of the follol ing Inolecules? u) sulfhldryls b) aldehydes ketones c) amjnoacids and d) sterols (R noneof these correct is MED 411 27. A substrateleyel phosphorylationstep that occurcin aerobicmetab;rlismr-,ccurs in? a) chemiosmosis b) alcoholic fermentation c) malateshuttle '.fr}Krebs cjcle e) noneoftheseis correct PRE ,A v MED 411 .CO prqortion (br'9.i, 25. The molecule(s) occun'ing the greatest in oftotal rveight) eukaryotic in cellsis (are)? a) inorganic ions b) sugars precursors, c) water d) macromolecr.rles noneoftheseis correct & e) COM Bil255s09Test#3 &Final 11. P - PR EM ED4 F orm2 ADP transport from the cltosol into the mitochondria occuls ria a co-tl?nsport mechanismin which t\. c) glucose rdlH- e) noneoftheseis correct b) ATP transporled along$ith ADP? a) NADH 11. COM is - PR EM ED4 in concertration ofcalciummight be expected I ,h" rute Krebscyclereactions'? Al increase the intracellular d) haveno eflectat all e) noneoftheseis correct a) decrease b) totallyinhibit Sincrease - PR EM ED4 11. COM processes components the breakdounof the cereal or in 3 o . Promamongthe givenlist of metabolic I'ou had for the ler pb1..sphur} breakfast today,which will directll generate mostATP by substrutc el '(dlarp htion'l c) pl mvatedehl'drogenase it nthase a) glycolysis b) xtebs cyclec,rmpLe^ ifnone ofthe aboveis correct e) choose this answer COM - PR EM ED4 11. COM r.hich rvouldreleasc greateramount freeenergl'?the gradualoxidationof the of 3r. Permoleofglucose, glucose duringcellularrespiration the rapid combustion glucose burning? or of b1' is by a) more free energy- released cell respiration by -b) moreenergfis released the burningofglucose '?) b1' exactll'the sameamount of overall free energy,per rnole,is released both methods d) choose this ansrver noneofthe aboveis correct if - PR EM ED4 11. while the final product ofanaerobic respiration is? 32. The final product ofglycolysis is p1'ruvate, c) DHAP e) noneoftheseis correct a) malate b) aceql-CoA I d) Iacticacid lbrceor chemical bond [hashmarks]depictcd 33. Theq-peof molecular iD the dra$'in!!to the right is most likely? b) disulfide bonds a) ionicbonds d) peptidebonds g) hl dlophobicbonds {e) choosethis ansu'erif none of theseis correct ED4 11. COM I i R_ - PR EM R_C_H c-olll I llll H- I c:ollllllll I c _H I I I H-N I FI_C_R I I - PR EM ED4 11. COM 34. fbe currentvier! ofthe role oftensegriq'incellstructurehasthe and tendons ant'lthe tensilervires madeup by the bones. /)-., runrpressionstrutsbeingmadeup ofthe ligaments \J la)rtrue b) faise I c H . o : 1 5 .A h l p , ) t h e t i ( u l r e d o xc o u p l e .M a l l e r a s e h a : 'i r s t : l r r ( L r r r l d o x p o t e n t i a L [ + o . o 3 r ' o l t sa n d t h e r e d x c r l r r p l e i O / O . COM rvill of -!as a redoxpotential +.8s volts. Electrons likcll'florvfrom? rd) Mallerase r.atel to b) from uater to Nlallerase is d) choose answerif noneof the above correct this - PR EM ED4 11. c) electronsw'ill not florr'at all 411 .CO MPRE M ED4 11. COM The reaction takingplacein the diagramto the right is classified as? 136. cellrrlar c) depollmelizition 6) a redoxreaction b; [r:dro\'sis e) choosethis answerif none of theseis correct 6) a carboxy'lation *r, - c t lta) -4.3 Kcal/mole, then the in 37. Ifthe AGo'for the reaction Qr4 is addition of more4qoductwill make this reaction go fasler? a) true r.b)lfalse MPRE MED Thc next 3 questions refer to the following answer choises: matrix C) cltosol A) nritochondrial membrane B) mitochondrial D) peri-mitochondrial space E) nucleus MED 411 .CO found in Eukanotic cells? complex 6 38. \{ hele is the p1rurptedehydrogenase c)E r)A b)B c)C d)D {) .CO MPRE leve 39. Whereis A liPprnred duringsubstrate I phosphorylation? d)D&E i a)A&B b)B&C c)C&D e ) n o n e f t h e s es a c o r l e cc h o i c e o t MPRE MED 411 -1u.Whereis the pH likely to bg*{relorr'cst? tdl\D h) B e) E ulA c)C PRE MED 411 .CO bondsare found in ploteins'? 4r. Whichof the followingchemical c) peptide bonds a) disulfide bridges b) hydrogenbonds /'6) choosethis answerif all of the aboveare correct d) hydrophobicbonds COM P a g e4 11. P ulzccsuv - tesl FJ &Frnal - PR EM ED4 F orm2 COM the next B questions are related 42. A o.r ml aliquot(i.e..a small fraction)of a 5.o ml homollenate (totalvolunc) ofa hurnankidneycells (weighing to.o gm) is shorvn havean Absorbance o.5o. Usingthe BSA to of (borineserumalbumin)Standard Proteincune to the right, 01 }tow much proteinis presen per gm o[ chir.ken t liver? ,.V rng b) zo mg c) zoo mg d) z.o g,m d ) n o t d e t e r m i n a b l e, r mt h i sd a t a fr - PR EM ED4 11. BSA COM 12 - PR EM ED4 11. 1 COM - PR EM ED4 11. COM converts 43. Ifthat sameo.r ml aliquotofkidnev homogenate rz pmoles glucose glucose-6-P 4 minutes, of to in then the total numberof dnits of enzrrneactivit) presentin the total xqlumehumankidney' cellsis? U 'a) 3 units b) 15units c) r5o units d) 3ooo units e) noneof theseis correct -3^^ b ". 2 < 04 0.2 0 COM - PR EM ED4 11. n f k 4 4 . T h c s p e c i l i c a c t i \ i o f t h ee n z ) m e r o m l h c s e i d n e v c e l l s ghlcose glucose-6-P converts to is? ;qhich 'a) o.75U/mg b) r.5 U/mg c) r5.o U/mg d) 15o.oU/mg e) noneofthese :,, d) electron transfer - PR EM ED4 11. and/or gather,energy which ol the follor,,ingmechanisms? by 45. Cellsare ableto captLrre t) crpture. light enerp' b]' pigments of b) redoxreactions c) hydrogen pumps ion / e l c h o o s eh r sa n s n e r l l l l r ' f t h e s e s c o r r e c t t i i - PR EM COM ^ \J - ED4 11. COM bondj3the chenrical bond that is mostresponsible a molecules 46. The covalent for ? a) conformrlion h) configuration c) eleclronegatiriI tl) size n,rn"oI ttrese corr"ct is ".1 Whilecellson Eirrthc.rnexhibitan amazing (bacteria lrrain)eachsharea cornmon diversih ofappearance 47. to bttilding block strchrts,:r a) presence nuclt'us b) rnitochondria c) prions d) presence a membrane of of e ) ( h ' ) o s eh r sJ n s r r c r t n o n eo f t h e s e s a c o r r e cc h o i c c t l i t ED4 11. the next 2 questions are related. MPRE MED 411 .CO MPRE M ED4 11. COM - PR EM dehldrogcnrse an enzl.rne is that oxidizes succinate fumarate. to 48. Succinate Malonicacidis an inhibitor ofthe elz)me SDH.A mitochondrial sample SDH gavethe fr-rllowing of data.By carefulexamination the data,determine of the probablethe Klmfor SDH ? rate of NATryE reaction rate ofinhibited reaction concentration succinate of 2.o unlts 2 . OU n l t s t.o pmoles starch of 7.5 unlts of 7.5units 4.o prmoles starch.l3.o unlts of 7 . 8u D i t s 9.r pmoles starch 15.o5 units 15.2 [noles ()fstarch 7 . 6u n i t s r.1.95 units z5.r ;tmoleof starch 7.7units s r5.o units 7.6 units 4o.2 lrnolesof starch .CO a) r.o pmole b) z.o ;rmoles cJ4.o pmole" d) 4o.2 lrmoles e) not determinable from the datathat is qiven MPRE MED 411 it 49. From the dataon SD/ above, lookslike malonicacid is workingaswhat kind of inhibit6r'/ a) competitive b) non-competitive c) irreversible d) non-permanent e) not deterrninable fronr the data that is siven MED 411 .CO 1- so.Jhe classof enzl mes t,ften involved in hydrogentransfersmost commonly are referredto as? \-/ r) transferases b) h)'dlol]'ases c) dehydrogenases d) ligases e) noneof theseis correct PRE MED 411 .CO MPRE ATP enz]me can frrnction eitherto slnthesize A'fP or pump protonsagainst ,., 5 t. The ntitoch,.-,ndriirl synthase ( b) false their electlor'h m icaI gradient by hydrolyzingATP to ADP + P. e a) trle \1 COM P age 5 11. P - PR EM reactiont1pe is classifiedas a _ 52. The follovv'ing a) functional group transfer b) hldroll'si.s 11. COM reaction: p r o t e i n r , + a m i n o i r , i d , < - - - > p r o t e i n , r , , r H . O , d ) c o n d e n s aito n c) redox t-)none(,Ilheseiscorrect ED4 I the next 2 questions are related _ Test &Final Bit255s09 #3 ED4 Form2 11. COM 5 'E0.4 o.r ED4 A 54. Whichenzy'me, or B, hasthe greateraffini\ for its ou'n ED4 11. COM - PR EM I o.7 o 0'6 Jlo E U.) - PR EM 0.9 .g 0.8 E 53. The Km for enzymeB is closestto which of the lbllowing? a) 2.5 prmoles -!) o.3 unrts c) z4 pmoles ?) 5.o pmoles e) noneof theseis correct ,0.2 0.1 - PR EM dub,strate? a.l'A b)s 0 11. COM c) neithcr.eachhasthe same affinity d) noneoftheseis correct ED4 1.5 2.1 3 4 5 - PR EM [S micromoles] 6 7 7.A I - PR EM ED4 11. COM 55. One of the most prevailingtheoriesfor the origins of Life on Earth is that the chemicalself-organization carbrrn of con:pounds leadto physical chernical conrplt'xitlrvhichbecame encapsulated b1'anamphiphiii.biloy". ond *,u. probablv progenitor all subsequent the of cells. a)true b) false COM group(s)inr oh ed in the disulfidebond is? chemical 56. The functional a) -POr b) -NH. c) CH. d) -C=O i:) noneof these - PR EM ED4 11. ofthe tissuc culturing of a human cen'ical cancercell; that cell is knoy,.n 57. Tlris year rnarksthe 58tr'anniversary as? a) squamous carcinoma b) Punkinjecell SHela celf tl) basalcell e) noneoftheseis correct rntl a) caroentoids b) specialized s8 4P..,68o PTooare? ch)orophllla molecrles c) both in ISJI 'tl) the onll chlorophi molecules ll that canabsorblight e) noneof thcseis correct 11. COM 6 V - PR EM ED4 5 q . L l c l i c e l ( c t r o nf l o u i n p h o t u s l n L h e s i s ? f \J c ) r e s u l l si n A I ' P p h o t o p h r , s p h o n l a t i o n a) involves onll'PSII d) isinhibited l CO2 b b)lresults Oz beineer.olvetl in e ) n o n eo f t h e s e s c o i r e c t i ED4 11. COM e-transfers occur? a) on plasmamembranc ofgreenplants C) on outerchloroplast L 60 Piotosvnthetjc membranes c) on th]lakold membranes d) on plant cellvacuole membranes e) noneoftnesers correcr .CO MPRE M (rr 6r. Porinis located? aJin outermembranes oflysosomes b) ip-uer membranes ofchloroplasts c] outer membranes rnitochondria d) in the roughE.R. of ie) noneoftheseis correct .CO 6 3 . P r o l o n s r c , t h o u g hu r f l c c tA I P s l n t h a s er 1 ? a tt l b)mo\lngthrotrghachannelintheFopar'1icle d ) c h r n B e sh e b i n d i n g a f f i n i 5 f A f l P & P i n F r s r r b u n i t t u MED 411 , tl, ' MPRE MED 411 62. Phosphofructokitrase, regnlatonenzlme ofgllcollsis, can be ellostglicalltresullted b\ ? a ke1 a)AMP b)ATP c)r'ilrrlc d ) f n r c t o s2 . 66 i s p h o s p h a t e/ J i r " i n r i g u l a t e d b v a l l u f r h e s e e a ) c h a n g i n g . t h e n f o r m a t i oo t t h e F t c o l n p o n c n t co n r o l r t i n g h ec - s r r b u n io f t h e F u s u b r r n i t t ts B) leMll of ltiese farc,rffects proton o of aclitrrrs MPRE f) 6:1.ATP can be madein the? a) mitochondria b) cltoplasm c) chloroplasm d) a and c onl1. a. b, & c arc correct MPRE MED 411 .CO b.5 Based upon the obsen'ation thatATP is present archaea-bacterir, in Eu$g1eria, pl.uts, and animals, one ma_r, concludethat? a) all theselife forms must contain mitochondria evolve<i an early life form in .BltrtR c) ATP wasoriginallv exlracellularwhich was endocrtosed d) all th$e life forms use fats as thei. .n".g1. .ou.." e) noneof theseis correct PRE MED 411 .CO 66. The protonmotiveforce?-a) is generated the hi tlrolysis ATP by of i) is generated ATp s'rthase by c) is driven by the ADT-ATP antipofi s]'stemin chloroplas'ts d) is in part a p.oton"undan giual"niO \ e) noneol lhesets correct "l""i.l"ut COM P age6 ED4 Form 2 - PR EM 'lhe nativeconformation a proteinis the 3D shape2$ichhasthe lou'est of freeenergy r:xpentliture forms and 'a)rflobular spontaneoull'. proteinsthis shapeis likelr'? l-or b)ahelix c)afiber d)atetramer e ) c h u o s eh i sa n s u e r f i t i s n o n eo f l h e s c l i - PR EM OS.t'he e'fe madein glvcolysis madebr'? is a) chemiosmosis b) r H*A1'Pase nre en4 phosphorylation d) oxidative b) substrate levelphosphorllation 6.!noneoftheseis correct COM fi - ED4 11. COM 67. Bil255s09 Test#3 &Final 11. P ED4 11. Part3. Professor Glaser Cumulative - PR EM 69.Telomerase requiredto prevent formationof a singlestranded is the DNA sequence the end of the replication at ofl a.The leading strand b. Thc lagging strand - PR EM ED4 11. COM find? 7o. Ifyou examinethe genescodingfor protein in the human genomeyor.r a. Mostofthe sequence represents exons b: N{ost ofthe seqnence represents introns ED4 11. COM (pickthe bestanster) nRNA codingfor 3 proteins cannotbe translated a rat liver cell,because'? in 7r. A bacterial u. It doesnot hlve a tnethl'lG cap ribosomes not-lecognize b. Eukaryotic do internalstafi sequences u./ c. Onll eukanoricproteinslnthesisstartsat an initiatorAUG d.'a,b and c apply e. onll'a and b apply COM - PR EM ED4 11. COM - PR EM that you havea DNA microarralthat allows}ou to identilr'zo,ooo differentDNA sequences randomll' 72. Imagine distributed across wholehumangelome. You usethis arravto measure abundance the the oftheseDNA sequences in humancellsgro*ing synchronously, all the cellsdir,ide approximately sametim€. Youexpect? i.e., at the a. The concentration all sequcnces increase the satnerate of to at b. The concentration somesequences increase to of earlierand otherlater You expect earlvsequences be closer the originsof replication the to to _c. (d. b and c are correct - PR EM ED4 11. (think caretulli) 73. AminoacllIRNA slrnthctases? (i.e.link an aminoacidto the IRNA) a. Rccognize anticodon IRNA & this is sufficient reaction proceed thc of for to b.'l-heenarnes also recDgnize other sequences the tRli.A. in COM The next two questions are related - PR EM ED4 11. proteins eukary-otes degraded? a. Itandornlr in are 74. Cellular a.'Ihe nucleus b. ln proteosomes COM retluires? 75. Proteinsare normally degradedin a pathu'ay;-qhat t.e.'b, and d appll c. ivlrrrked ubiquitin by d. ATP c b. Differentproteinare degraded differentrates at 411 .CO MPRE M ED4 11. ("1C) reducecysteinc(labeledrvith 'ac) Iinked to its IRNA to Benerate labeledalaninestill linked to 76. You chemicall.v the IRNA. You usethis as a substratein an in uitro protein synthesisincluding all normal aminoacyltRNA's ribosomes etc.You usean artificial message coding sequence: of AUG GCAGGAAGC LIGCACA GCC You expectto tind ('+g; alaninein tlpgesulting pollpeptide in? 1 2 6 3 4 S 7 2 a. Position b. Position c. Prrsition d. a.lnd b apply 7 5 .CO MPRE MED 77. E.colicellslacktelomerase thel'sunive because?'et, reproducc rapidlyin largenumbers and caDtolerate multipiemutations ,1. They1 b, 'l'heir DNA has no ends c. A deoxlribonucleotide triphosphate MPRE MED 411 is 78.ANA s\nthesis al\vatsinitiatedb] ? a.A RNA primer b. A guanosine cap 411 .CO 79. The RNA primer of eachOkaz+i fragment is? ' b. Degraded indiyidual nucleotides a. Releaserl and reused to PRE MED 411 .CO MPRE MED 8o. \\l-richof the folkrwing sourcesof "Stem Cells"would be the most desirableto cure a "liver defect"in an adult human male? Think very carefull]: considersourceonll', but not the difficulq in obtaining the cells. a. Stemcellsfrom Ieft orer blastoosts after in ritro fertilization b. Stemcellsfrom nucleartransfer using the nuclei from the patientsfibroblasts c. Stern cellsobtained genetic bl changes the patients in fibroblasts d.,band c rvouldbe ok e. a, b and c wouldbe ok COM P a g e7 11. P B i l 2 5 5 s o- T e s t# 3 & F i n a l 9 - PR EM ED4 F o r m2 ED4 11. COM 8r. The driving forcefbr Na' to movetiom the extracellular fluid to the cell interior is due to: a.The difference concentration in vs. Na*out of Na* b. The magnitude and polarityof the membrane potentiill c)aandbapply - PR EM ED4 11. COM - PR EM 82. tRNA synthases havea proofreading function.A mousemutant in ra'hich tRNA synthase tRNA alanine. the for will also relativelvinfrequently recognize serineand link it to this IRNA. The mutant miie doesnot qrorvrvell and die at an early age.You examinethe sequence someproteins from the liver and you expect? of a. to find alanine positions at that in a normalmousehasserine b. Find serineat positions that in a normalmousehavealanine c. both a and b appl1. COM 11. - PR EM ED4 $ S3 Amplification ofa particularDNA sequence Polymerase bv ChainReaction requires knorvledge ofl a. The full sequence the regionyou wish to amplifl' of b. The sequence ofthe 3'and 5' endofthe regionthat ).ouwish to amplif; c. A specific restriction enzy-me d. a and b apply e.b and c apply - PR EM ED4 11. COM 84. Youu'ishto determine function- a gene(UU) rvhichis expresserl manvtissues the of in includingin the mammar\. glandcellsthat secrete milk. Lactalbumin a proteinthat is onl.l secreted nrilk.In order to i-nactivate gene i.s in the (UU) in milk secreting cellsyou nould mateanimals ith the followinggenetic n (I, modifications II, III, IV ari exons in the genefor protein U) rvith an animal cxprcssingthe geneCrc undcr control of an inducible promoter activated b1'administration an antibiotic. of Hexokinase a proteinexpressed mostcells? is in ED4 r-,r_l E x I .", I I ur I I rv I'\ "' LfJ-I r-"x lL I - PR EM O 11. COM ")L4-l'"" LL__J | t4 I l'* Creuith Lactllbuminpromoter " E;I-F_-I Creu'ith antibioticpromoter - PR EM ED4 11. COM .,8_E E_E Cre u'ith hexokinase promoter - PR EM ED4 11. COM 85. Proteins associated with cellularmembranes suchasthe o,toplasmic membrane? a . S o m e r el i n k e dl o t h om c m b r a n ei a f a t h . a c i d a \ p15," menrbrane an crhelix h. S,'lnp 11x\ the as c. Someattachto the surface ofthe membrane hydrophobic interactions 'e.), by d. a, b and c are correct b are correct MPRE M ED4 11. COM 86. The Ca'. ATPasc? (pickthe bestansrver) a. Transfers Ca" fiom the ER into the cltoolasm h . ( n l r n s p o r l a i n t o t h eE R r r g a i n s its c o n c e n t r a t i o n a d i e n t C i gr {12 \ c. ('an transpurt Ct** from the ER into the cytoplasrn againitits concentration gradient 0. a ano c arecorrect 411 .CO MPRE MED 411 .CO 87. Intestinal epithelia have? a. Facilitated diffusion(transpon)for glucose their apicalmembrane at and a Na*/glucose s]'mpoftat their . basolirteral membrane b. !acilitateddiffusionfor glucose tlansportat their basolateral membrane ancla Na*/glrrcose slmporl at their aplcal c. Neithera or b are correct .CO MPRE MED 411 .CO MPRE MED ', 88. Sone cellsIinkedto adjacent cellsbr' "junctions includinggapjunctionsand tight iunctions.Whichof the statements belowbestdescribe iunctions? the a. Gapjurctionscreate channel a berw-een rvhichallowsmolecules cells with molecular weight<13ooto trayerse from one cell to the other 'Iightjunctions b. prevent diffusionof membrane proteinbetween basolateral the membrane and the apical membranes c. In theabsenceof tight junctions, traniport ofglucose tiom the intestinal lumen to the blood woultl be ineffective d. a andb are correct ie.:a, b and c are correcr PRE MED 411 89.*Vhenstemcellsin-aparticulariissuedivide,the mostlikelyoutcomeis? a. Both daughter cellsare stemcells b)One daughtercell remainsas a stem cell and the other initiates a path of further dilisio-nsand differentiation COM 11. P Bil255s09 Test#3 &Final ED4 Form2 Page8 ED4 11. COM - PR EM qo. 'l'he boundan betwcenexonsand introns is? 6)Frecisely'definedso that splicing alwavsoccursat the samesite b. The boundaryis variable and splicing canoccurin manl'regions the sameexon/intronboundary for - PR EM ED4 11. COM - PR EM 91. Doublestrandcdbreaksin DNA canbe lepaired by a non homologonsmechanismor homologous reconbination? a. Repairbl homologousrecombinationresultsin lossof geneticinformation b. Repairby'non homologousrecombinationresults in lossof geneticinformation ' gociq, i.e.both homologous c. Homologous recombination results potential in lossof heterozl chromosomes har-e the samesequence the siteof the dpublestranded break,afterhomologous repair at .6b anclc are cc,rret:t d. a and c are correcf COM 11. ED4 - PR EM b ofseveraltranscription a. Permanent expression factors to 92. To converta skin fibroblast an iPScell,you need? factors -c. Reversal ofepigenetic chlnges b. Tlansientexpression ofseleral transcription G. a and c apply d. b and c appll 11. COM whichcan mole overevolutionar,time,)ou expect? elements the humangenome, in 93. L1 are transposable effect a.. The movement no biological has -tr T h i sm o r e m e nc a nr e s u l t n a m u t a t i o n u c ha sh e m o p h i l i a i s t COM - PR EM ED4 the 94. Most amino acidsare codedby multiple transfer RNAs (tRNAs). You expecta mutation that r:hirnges codeword f9-r fbr a particular aminoacid (i.e. the nerccorleword in the mRNA codes the sameaminoacid)to? in a. Have no biolopiical affect b. Resultin an altered splicing pattern i,rBoth a & b ma1'appl1, different proteins - PR EM 0 ED4 11. reaches end ofthe codingregionofan mRNA? the 95. \\hcn the ribosome the b. It releabes free pol)lg.ptide the a. It releases polypeptidechain still linked to the last IRNA /e\b and c apply tl. a Lrnd appll c c. Thereis a specific tRNi\ that bindsto the terminationcodon(s) - PR EM ED4 11. COM triphosphateand RNA pol)'merase of a. Four ribonucleoside 96. S1'nthesis RNA requires? site c. Regulatoryproteins that control transcription b. A promoterand an initiationof transcription '/ e. a, b and c apply d.aandbapplia. At randomsites d. a and c appll b. At nniquesequences ' re. b and c appl]. 11. COM DNA? enzlmescleave 97. Restriction c. The sequence the siteis oft€na palindrome at 98. Ribozlnes are? ( pick the bestansu'er) r'r'ith b. RI,r-A-s catalltic actilit! d.aandbapply COM - PR EM ED4 that cleaveribonucleoticle a. Enz-!mes ,c.En4 mesthat cleaveRNA at specificsequences and c app\' .'e.\b MPRE M ED4 11. 99. A cDNA library is lyepared from musclecells& Iiver cells. You expectthe genesfound in thesetrvo libraries to be'? ' b. Partially overlapping c. Totalll different a. The srrme 411 .CO loo. Retrotransposons from onelocationin the genome and moveit to anotherlocation. excise DNA sequence a b. felse a.True MPRE MED 411 .CO MPRE MED 2nd base in codon U U c .CO 411 MED MPRE .CO 411 MED PRE n p A G Phe Leu Leu L€u Leu L€q lle lle ll9 Mot Val Val c Sea S€. Ser Pro Pro Pro P(o Thr Ttr Thr Thr Alr Ala Aa AG cvr Tyt cyt Tyr STOP sTop STOP TrI Hi3 H;S Gln Lys Lys Asr Astl Gl{ Clu A,s A'g Ar! aro SDr Ser ar{ arq Glv Gtv Glv U q U U c U c o COM - PR EM ED4 11. COM - PR EM ED4 11. P I C O N S E N I I O H A V E t ' ] ! YG A A D E P O S T E O B Y M Y ] D COM NUMBER CI.]A CLASS AOSTEI] 11. SIGNATUFE DATE HAVE I.IEITI,iEI] ]VEN NOII I]ECEIVID AIO ON THIS TXAMINATIOIJ G - PR EM ED4 oI\I MY HONOR. DE ABCOE .,_ o 2 iil(r-r!1 ABC zt L" 3,Q ij; f1l 11. DE AACDE ABCOE 11. t6 O (:) iL) ..,,'.isi :t AACDE IJE i7 i.rOOr.ja-1, ot COM 11. ED4 js ii,..! '1.,6tt. - PR EM i.1,, AFCO' .CO ABCDE MED ABCOE 411 55ai (i) aj)J lr.i MPRE 55 ij,' r-a- ti C l:li , r ,:- 411 .CO AACDE AACOE MPRE MED ie't Ca,r(ili. AECOE .CO 5 si . t , t l O j i l . r AsCDE MPRE MED 411 60ri :,1l. i.ii- .CO COM ED4 ABCDE .) 52r1-' .-,i!. riD .2.: COM 11. ED4 MPRE M AACOE s . lQ O r i : ; i r ' , i 411 l 3 0Q a ?r l t r : i r ' : , 1 AACDF lZ l-;: q'-:-r iO I ,' !) ABCOE ABCOF 63 'l O (-r.i.;,ri.5-: 73,,i_)O(i (;- r5r ABCDE 64 a (,_) i ai),3r C] ABCDE (t(Drit(i, 7,1 ABCDE 6 5i j - r O l ' i l . l AACOE 6 6l t r , 3 € - ) O i r l ABCDE ABCDE 76,l ar c'i. ill ABCOE 77 .,,r i.r),:{,, ir. O AECDE 6 s- ! ) O ( j l l , - r , . ' l ASCOE 6sa)Oij_r(ll rr. ANCOE +eL.rCi, : .r. AACDE r.r i-rl 3 7 i - , ' lO , : t ',. lr i. rr, O,: rB,,)Oa li . 3 si o l r ABCOU AECDE {0,;,Iti'O s1 ,',.L Qir aaci)t :t;C'lt ..1 r sr i , ! $ ; i . . i , 5o'f Ot l ct 1.,: .ffG AACDE s z , O , - . , rr , . S? r :r_.t C. aacof Bl ,.,, (D i:l i:r. ''r : ,!Dit,.1,, e3,.1 AACDI B,ri il:t|Oi.;) e a , . ,4 r e i - , AACO€ 85.t) ii ':J:f G AACDI rD s6-;-) ar .:. .' ABCDE ASCOE el1,,!i,,,i ' AECDE -j s61r '?'-, O ABCC' s7'r,Qia)'ri .,AACDI: qj al ,. -.t r G ABCDE ABCOe es.,A.t. t , 4ALL1 ABCOE !r0rGi.:r AACDE AACDL i;'l ec qi., l:1 r-, r f.r AACOE 'i AACDE il . ABC95 79ri,4]rr,1r'. -r')ia:r'rj LCt; AB'DE r.q A8',DE il 8 s . L : ' i . r. ; , O B0 AACOE ABCOE a il rsO.t..)'i:, ABCOE 70r.t,O:ll(j'(:) .t 15ilr a, Jl r'i O ABCOE 67{D (!l (,) i.ti (' ) AACDE 3 50 : , i j - : - j r i 5 ) AACDI 7 1( : : - : i r - r i f O ABCDF ABCDF ABCDE raCt-ii irr. AAC]]E AACDI 29 ir, i.:l r3 il' ,rl 11. aBcDf AACDT bl .i .r.,(l4 - PR EM ,:Jr:rl 52i rr,',fl)O ., MED AACDE ABCOF 2 0 r , , i - r - rrr a ABCDE i3 .1,:-?.r O..,, t,,, l:l Ll, r.,f ..r'r,r- ABCOE 2B ,e J,,ir)ij. it_ ABCOT s t0 O . j PRE (t 2i l-'r ir- a.i) r-'l ANCOF t o ' i r r r - tf i l D s7 ii ) i:'r ,l ABCOE A6COE t8 !i;r,l!fr'r,Q /rf,{ tr-, AACDE 33 r:,, it-t i:'.)tr.rO AACOE -,,26a:rC .r) ''t' ASCDE Bi t l O a l ot - r,76\ c .:.t ABCI]E ar E 32(t.a) t:..(D\:". 12J. Q.,_,.,,_ ,_ Ailc0E AACDE - PR EM 7 (t_-r r-t)(r.,:,:-t O ABC COM ABCDE OE ABC A6C i5 i,) /? alr er,r,, zs(.'_:Q)Qr,9tli| 35O ia,r:r i1t, r_ .1rii at, :irrj O ED4 AtsC .l-:,:.{E AtsCDE ED4 ABCDF ll AtJCDt ASCDE - PR EM DE r-".1 5 a.rl.:-)O ali 6(rr,t, i j o) ABCDE 3 1 : t _ , 4 ! _ ' o1 ri : ra(.)a.t)il)Ca) z q . : r € l i ] r O i : r ) 34at)O ar)itr \:l { (rti!) (J !- a' ABC ABCDE z: r1)t?:Q L1 (ir 11. DE COM (D.tl r (rlO.r i:) a:l i3 ,J €) a-,, ABC AACDE 22::. 't_r(i,r(JlO 12ail .al, rOiii all DE ABCDE COM 11,.f (t.!)..i) i ED4 AAC '!:q S I D E1 AECCE - PR EM 1 i., a3t i.l ABCOE AACJE r r. € { r , i r iC t I l tlIti.rO-. : ...
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