Test 1 Spring 2006

Test 1 Spring 2006 - / ". @ ejO’lJmmS > f "f...

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Unformatted text preview: / ". @ ejO’lJmmS > f "f ’ Bil 255 SecTion Q l) A, -:.. (\mwfill't l/ Q + l TesT #1 - Mallery . ' ' FORM 0 fix EDI/25$ Feb 23‘ 2006 Spring 2000 F) DIRECTIONS: Ch se The BEST answer from among The choices given. i\ in?“ "W g/do N26 m”? V . F he reacTion A -——‘> B To occur exergonically in cells ? f Xi. ,. 3,. filepiie Nfi‘f . ' J a) musT avea b) The reacTion musT be coupled To The condensation 0f‘ADP and P ,9 O - c'fiJa ree eWMWWe N {H 0 w) l KQ e A6” of The reacTion musT e>(+) e) choose This answer if none of The above is correcT lg, _ Tabolic paThways function in cells primarily because of? '40) coup d reacTions b) all r‘eacTians are exergonic c) presence of 6—proTeins (GTPoses ropy is always losT e) chOOSe This answer if none of The above is correcT . Of The six major Types of key meTabolic reacTions in cells, The following is an example of? glucose—glucose“) + H 20 <--> glucoSe-glucose{,.-n f \I a) oxidaTion reducTion b) condensation c) isomerizaTion db/hydrolysis e) none of These . The cell mosT commonly uses which of The following reacTions To bring abouT biological order by linkin}a Thermodynamicolly unfavored reacTions wiTh This Thermodynamically favored reacTian? "-x a) hyd/ifilysis of faTs (chondensafion of ADP d P c) hydrolysis 0&1? d nThesis of glucose e) choose This answer if none of The above is correcT \u .‘ \ 05 f The sTandard free energy change for a reactions aT equilibrium is calculaTed To be negaTive, Th The acTuc ' free energy ch ge of This reacTion in cells musT always be negaTive as well? a} True @alse « .- protein standard curve The nex quesTions refer To The graph below B researcher homogenizes 0 5.09m sample of ain Tissue in a blender wiTh 10 ml of buffer. The ToTaI sample is TreaTed via differenTial cenTrifugoTion and afTer 010,000xg spin, The liquid porTion of The samp is collecTed’25i\_is referred To as The? pelleT~ supernaTanT ‘ cHracTian . d)‘chromaTography e) none of These is correcT 0 06. T. 0.20 0.40 0.60 0.00 1.00 120 1,40 1.! mdabummpratoin 7}, A small sample (0.1 ml) of The liquid porTion above is mixed ‘ ( wiTh Biur reagenT for a proTein assay di reTurns an absorbance aT 510 nm of 0.4; how much equivalenT albun n is presenT in This sample? a) 0.4 abs/min b) 0.15 mg/ml = (“OWE mgfml (B .lm loi ml d) 1.6 rag/Jul Aj e) none of These Id porTiofibove has a volume of 15.0 ml, how much ToTal proTein would be presenT in This 5.09m 1 8:01: The li ' a 135 mg b} 60 mg c) 0.16 mg d) 0.9 mg e) none of These is correcT /’ sample ain? es work by lowering a formgj free energy known as? a) exergonic energy ) endergonic energy -:g,ocTivaTion energy cl) vibraTionai energy e) none of These The major- class of enzymes ThaT are responsible for reacT' __s involving coenzymes as NAD' 6i FAD are? a) ligfiw b) Transferases W5 "d I l idoreclucTases e) none of These ' l Bil 255 $06 — Test #1 Form 0 — 32306 pg 2 glucose phosphatase family mily of glucose phosPhatases to the right, what is the Vmax of enzyme '6" ? a) 24 umoles/min glucose b) 16 umoles/min glucose c) 24 crpmoles 6—6-phosphate d) micromole 6-6-phosphate none of these is correct _ l,__ Approximately How many International Units ofacfivi are shown by enzyme “A” '3 05 M M 2 4 a w IQJ/G C) micromolos G-G-Phnlphlt. O IU e) none of These is a correct choice i UM!!! / / What is the/Km for The enzyme B ? a} 8.0 umoles 66P b) 4.0 umolesGéP 2L4 c) Gymoles 66F d) 12 umoles glucose e) none of these is correct fl ' " .-—--"‘ __ , Rfié/finn/zymelcarve 'C“ represents an inhibited version of the native curve CA"), then the of in n mhibiti being shown is? a) irreversible b) competitive __ on—competitive d) covalent modification e) not treatable by Michaleis-Menten kinetics e activfiof an enzyme which _is_e><pressed as the number of umoles/min/mg protein is the? a) international activity (.b))specific activity c) molecular activity d) none of these ./.. ' \ I F‘\ - We number of substrate molecules converted to product per second for a single enzlymewnolecule is known as the enzyme's? turnover number in) the half-time x c) rat I enhancqnent - d molecular activit e none of these is correct .' -“ - ' b, ) Y ) tun L J h 1 Answer the next 2 question with references to this figure. ‘ Which of the following is the best choice for the highest level of pro in A“ structure being ibited by this enzyme? a) primary secondary c) tertiary cl) quarternary “ valent weak electrostatic force is exhibited at "C'Tfi/ __ ,_., _ on w b) hydrophobic interactions -"3d)"_'dipoles e) none of these \x": l m ich type of émtrifugation would separate subcell component's over a continuous shallow gradient sucrose? 0) SDS-PAGE @velocity sedimentation I cli ical centrifugation d) CsCl centrifugation e) choose this answer if none of these 5 correct ' r size is? a) ion exchange (Bigel filtration c) affinity d) none of these 21 P teins that are separated in a gel of a continuous pH gradient and move to point in the gel equal to the protein's pI are being separated by? /a) P E 1:} SDS-PAGE isoelectric focusing d} HPLC e) none of these is correct \ . Most of the metaboiic reactions that occur in cells are at equilibrium. 0) true flalse Bil 255 506 — Test #1 Form D - 2123106 [3 ype of gel elecTrophoresis ThaT can separate proTeins on The basis of Their molecular weighT is? PAGE §D.5:PA&E c) velociTy SedimenTaTion d) HPLC e) none of These .1. I. ich of The following proTeomic Techniques would besT be used To deTermine a proTein's BD-shape? _ a 13W @X-ray crysTallogrophy "h e none of These Techniques would work 0‘ L i ilk . A’primar‘y reason ThoT cells are ofTen homogenized on- ce is? ../'a) celis are grown in Tissue culTure aT 4°C b) To mainTain an isoTonic medium revenT proTeolyTic digesTion dTTo‘sTayrcool-onlniotsummeedays-iW oose This answer if none of These is correcT c) T e cenTrifugaTion Technique ThoT would be The easiesT To isolate chloroplasTs from oTher cell organelles mo: ikely would be? “@ifferenfial cenTrifugaTion b) HPLC c) equilibrium sedimenTaTion ' ' ' 'on e) none of These I .._;.- biqui ‘ mediaTed proTeolysis requires which of The following molecular machines? I choperonins l5) proTeasomes c) GroEl + GroES d) ribosomes e) none of These '\ m if: . The naTive conformaTion of mosT enzyme proTeins? :3) is obular b) has an exTerior pockeT of hydrophobic amino acids chflhfidfie d "as an inTerior surface of hydrophilic amino acids 8) all of These are correcT _ high of The following proTein(s) is(are) noT needed for proper folding of many cellular proTeins? a) iquiTin b) HSP-TO c] GroES d) GroEL e) all of These are needed 0 AbnormalIy-sTrucTured forms of a proTein capable of converTing oTher proTeins inTo abnormal forms which are re5ponsibie for diseases classified as TSE's are known as? a) HSP-70's b) ubiquiTins c) choperonins d) prigns) e) none of These is corré‘; . A TerTiary level of proTein sTrucTure ThoT forms a compacT folded region and is ofTen self-forming a self- sTabilizing, and which is found in a varieTy of oTher proTeins is known as ? moTif a domain c) chaperonin d) proTeasorne e) none of These is correcT ruvaTe dehydrogenase (fig To righT) is a moiecular machine, made of 60 individual polypepTides of 3 differenT enzyme proTeins and 5 differenT coenzymeS. IT exhibiTs which levels of proTein sTrucTure? a) primary b) secondary c) TerTiary d) quarTernary choose This answer if PDH exhibiTs ali of These levei of proTein sTrucTure shown in ' The figure To The righT is mosT likely? a) secondary TerTiary c) quarTernary d) a domain e) none of These aT vary in primary sequenceihul have The same caTalyTic funcTion or '3 mains b) polymorphic e) none 0'? These e pepTide band? a) is shorTer and sTronger Than C-C blis longer, yeT weaker Than (526 c) allows no free roTaTion d) resulTs is zig—zag planar molecule 'e) all of These are correcT - -. i“ I { ~ __ ‘Bi1255306—Tesut1 FormD—ZIZMOBpgA W 'I . .k_\ . Thelikeiy charge of the amino acid to The right at pH 4.5 is? ' LCHQ—FH—COOH i. I \ N a) 0 b) -1 c) +1 d) +2 e) none of These 0 e, offal 4.5, would be classified as? b _b polar uncharged cmn—pola: d) aromatic e) none of theSe if e amino acid n is The shape of a molecule, which may not be altered without breaking bonds? a) true @faise _ a) combines two molecules with the concomitant loss of water nsfers a phosphate from ATP to glucose c) forms a peptide bond ' emoves electrons from a donor Transferring Them to an acceptor e) none of These is a correct choice owl-he forces of Tension and compression balance I c) celis grip the surface'ofa'glfiss‘li'éfitdish e) choose This answer if all of These are correct ovalent and non—covalent electrostatic forces control molecular shape and form. resulting in The forces of c figuration and conformation that produce biologically active molecules? '6) tlr‘ue b) false 4 The ma er composed of a nitrogenous base, a 5 carbon sugar, and a phosphate is? --—;——_J ' Wot b) a nucleotide c) a lipid d) a protein '<' e) a polysaccharide "Er it (“C-C ‘ e functional group w ‘ h makes a portion of fatty acid molecule on acid is its ? droxyl group ) arboxyl group c) phosphate group d) sulfhydryl group e) none of these . e observation that cell structure and function are similar from organism to organiSm, despite differences in cell specialization and organism complexity provides circumstantial evidence That? a)) all c ls have evolved from a common ancestor b) cells do not evolve -——~ d}oH~cellshave‘fhe"scifia‘bifiéfiifit‘ffi~_rrépro‘dfltfionw ate of abiotically produced chemically reactive molecules is often referred To as a? 9) roteasome b) p‘rotobiont c) coacervates d) tensegrity structure e) none of theSe r” ' e most significant property of living cells is their autonomous replication? ca) frue b) faISe analism b) Panspermia c) VitaliSm d)¥_SE'l'~I".§ ’ e) none of these is correct ory replaced the mainstream scientific thought of 17th century, which was? vancpfnent in the early history of cell biology was the ability To remove biological reactions from ’Test Tube? a) True b) false Th; ny as ects of biology are similar in most or all organisms, however it is often easier to analyze a part ular process aspects in a specific organism. Such organisms are often known as? a) uinea pigs b) viruses c) model organisms d) always plants e) none of these is correct e concept of trying to interpret The properties of a living organism by a detailed study of its individual onstituent molecules and their individual properties may be referred to as ? a) Vitalism b) Natural Selection _ "'c) J ductionism d) Micrographia e) none of these l mt. M We: 4 ‘2' Bil 255 82006 — T2 — Glaser - Form 1 — pg 2 l5. In prokaryotes the synthesis of enzymes required to make essential amino acidsfvitaminsfsugars depends on the availability of these in the fluid in which cells grow. Repressors are best described as? a. Proteinsthat controlamrrimrUptakeJechells— l. Proteins that bind to DNA at promoter regions, dependent on concentration of specific ligands in cells, for example, an amino acid 0. Are a component of RNA polymerase 16. You have discovered a new gene coding for glaserase a protein required for survival in B10 255. You obtain a DNA fragment with sequence... AUGCCUAAGGUA While you contemplate what to do with this information, one of your “friends” steels the sequence and tells you that from this he can determine the amino acid sequence of Glaserase and prepare an antibody to it by synthesizing a small polypeptide coded by this sequence. What is the sequence of this polypepticle? 3. Met - Pro - Lys — Val b. Cys - Leu — Arg ©Y0ur friend has no clue and can not do this The ne t two questions are related 1?". 11 proteins in a given cell type are degraded? . Randomly Different protein are degraded at different rates 18 roteins are normally degaded in? a. The nucleus b. In proteosomes c. Marked by ubiquitin d. a and 0 apply and c apply 19. H an females have two X chromosomes while males have one, therefore females? a. Synthesize twice as many gene products coded by the X chromosome b andomly inactivate half the genes on each X chromosome . Inactivate a specific set of genes in each X chromosome, so that each chromosome only expresses a ( different half of the X chromosome genes 7d..:l\-'one of the above is correct / , 0. Starting at the origin of replication, the replication forlfis) move(s) in? / a. One direction b. Two directions ‘1 DNA polymerase proofreading? ‘ a. Results in its dissociation from the DNA if a mistake has been made ‘ . emoval of the mismatched base and its replacement by the correct base c. Cleavage of the template strand 3. Four ribonucleoside triph05phate and RNA polymerase b. Promoter and an initiation of transcription site C. Regulatory proteins that control transcription d. a and b apply a, b, and 0 apply RNA is synthesized by copying DNA? a. Both strands of DNA are transcribed to yield double stranded RNA 915?. At any position in the genome only one strand of DNA is transcribed 8/ 22. S7hesis of RNA requires? . .RNA molecules have? a. No secondary structure b. Fold at random ; chan fold using base pairing rules into unique structures Bil 255 82006 — T2 — Glaser - Form 1 - pg 4 2 . The acetylation of histones in the nucleosome generally? a. Promotes (facilitates) gene transcription b. Blocks gene transcription _ 6. Helicase IS required for DNA replication. It? g/aT‘Unwinds the DNA helix b. Unwinds the DNA helix in an ATP dependent manner c. Neither statement is correct / >{Dij/following proteins are required for DNA synthesis? a . DNA polymerase, p'rimase, sliding clamp {q Helicase, DNA ligase (QRNA polymerase ’ d. and b apply and c apply " \ZE/ U acil is a base unique to RNA; it pairs with? a. Guanosine b. Cytosine EiJAdenine 'bosomes are best described as? a. A large set of proteins and DNA fr" b. Two particles; the large and small ribosomal subunit each containing RNA and protein c. Particles containing an RNA which can function as an enzyme (catalyst) ‘9/ . a and c apply 63 b and c apply . given messenger at any time will havE-L‘attached to it? a. One ribosome at the end b. One ribosome at each end gMultiple ribosomes in the process of synthesizing protein . 1 T . e minimal components required for function of a eucaryotic chromosome are? a. Centromere b. Replication origin c. Telomere and telomerase All apply ' __ 1. -~ X/DNA ligase requires (pick the best answer)? a. An RNA primer rib. ATP ; i . "\3/ T sposons are? a. Enzymes that cleave DNA b. Viruses “:03 DNA sequences that move to different locations on genome "‘ . A gene contains 6 exons, a mutation results in a deletion of the last exon. You expect? a. The synthesis of a shorter protein ‘ b. No protein is made Q1. Synthesis of a shorter protein if exon five has a polyadenylatiOn signal y" d. Synthesis of a shorter protein if exon five has a signal for the synthesis of a methyl G Cap a You clone a DNA fragment in a plasmid using EcoRi cleavage of the plasmid. This DNA is expressed in E.coli. It codes for an enzyme in amino acid metabolism. You have no amino acid sequence or DNA sequence for this gene, but you wish to obtain the DNA sequence. You have a full DNA sequence of the original plasmid. The restriction map for the plasmid with this gene inserted is: original plasmid Wt, inserted gene Hind III /' '\ /‘ '\ Not 1 EcoRi EcoRi In order to sequence this DNA you would release gene from the plasmid with? (think carefiilly) a. EcoRi b. Not 1 c. Hind 111 (d. Not 1 and Hind III e) none ofthese I / Bll 255 32006 — T2 — Glaser - Form 1 — pg . Higher organisms often contain multiple proteins which have apparently evolved from a common ancestral gene. ,The most likely origin of these is? éaflUnequal crossing over b. Bacterial infections chtion of enhancer molecules close to the initiation site of gene transcription xiii-"A p cular set of “leucine zipper" regulatory proteins is composed of three different chains A, B, C. Eacl can form homodimers or heterodimers with other chains in the set. How many different DNA sequences c this family of regulatory proteins recognize? a. 3 b. 5 @6 38. o amplify a particular region of DNA, by PCR you need: ' a. Knowledge of the nucleotide sequence at one end of the region you wish to amplify b. Knowledge of the nucleotide sequence at both ends of the region you wish to amplify} c. DNA polymerase which is femostable and all four deoxynucleoside triphosphate u d. a and 0 apply Lia/"b and 0 apply gé Messenger RNAs are degraded in cells? (pick the best answer) " a. All messenger RNAs are degraded at same rate “(6b. Different mRNAs are degraded at different rate “c. The 3’UTR (untranslated region) influences the rate of mRNA degradation. Stem cells are maintained because when they divide they undergo? j a. Asymmetrical division b. Daughter cells are subject to environmental asymmetry oth apply \ 1 fig ult stem cells are progenitor of? ' a' Only one differentiated cell Can give rise to multiple differentiated cells in the same tissuer’organ. «lg. Stepoid hormones, for example cortisone, control gene transcription by? I a. Lnterealating in DNA and distorting the helix -' b. Binding to a specific steroid binding protein that acts as an RNA polymerase - I e._Binding to a specific steroid binding protein that binds to an enhancer sequence for steroid sensitive genes 4%. You wish to clone the gene for red hair, so everyone can look like Luis Glaser. You determine a partial amino acid sequence of a protein that determines hair color as: Ala-Phy—Gly—Gly-Leu and after looking up the genetic code (attached), you synthesize an oligonucleotide probe to identify the gene from a genome library. The oligonucleotide that you synthesize has the sequence \ GCC-UUC-GGU—GGG-UUA d after five years of work you fail to find any sign of the gone. You should have? ,. a. Come to class more often b. Synthesized a series of random oligonucleotides kc. ynthesized all possible oligonucleotides that can code for the amino acid sequence above \../ . The carboxy region of RNA polymerase II is phosphorylated before the enzyme starts to transcribe DNA. The function of this is to? a. Provide a negative charge so the polymerase does not bend tightly to DNA Kb. Provide binding sites for the enzymes that modify the primary transcript, so that the processing ofthe RNA to form mRNA occurs contranslationally. Bil 255 82006 — T2 — Glaser - Form 1 — pg 5 The next two questions are related ~45. You want to develop a “genetic cure” for a decease that is the result of a mutation in one specific gene. You clone the gene and insert it into a retrovirus which has also been engineered to not reproduce in human cells but can infect these cells. The inserted sequence should minimally include? . a. The full sequence of the gene including introns and exons b. A binding site for transcription factors and RNA polymerase c. The full set of regulatory sequences for tissue specific expression of the gene .-l d. a and c apply a” F e. a, b and c apply f 456k} You wish to create a transgenic plant (corn), resistant to the weed killer Roundup, but do not want to risk the new genes being present in the edible portion of the plant, so that when Glaser eats tortillas he is not exposed to new proteins or genes. Which of the following genes insertions would accomplish this task? (symbols are the same in all cases). (Cre) Roundup LoxP Recombinase Resistance Corn Kernels Gene Specific Promoter Promoter Active in all tissues ems—m— . LoxP (Cre) Roundup LoxP r Resistance :7 following two questions are related: Two children are born in a hOSpitai at the Uni’versity of Florida. Since Gators are not careful, the blood samples taken from the two mothers may have been mislabeled and there is a possibility that the parents took home the wrong child. Before alarming anybody, a bright UM graduate suggests a RFLP analysis (DNA fingerprinting) to see whether she can solve the problem. The results with the first probe are: r Parents A Child X Parents B Child Y Mother Father They took‘home . Mother Father They took home 41. Child KW @Child Y does not belong to Parents B i" 0. One child could belong to either parent 0 a and b are correct a and c are correct Bil 255 82006 — T2 — Glaser - Form 1 - pg 7 ‘ The origin of different hemoglobin chains, for example, a & 6 in adult hemoglobin is likely to be due to? Unequal crossing over in an ancestral gene b. Differential splicing Q Exon shuffling d. none of these is correct \. A The experiment in previous question was repeated using a different probe for a different region of the genome. (Think carefully) a. The new probe can confirm or negate the positive identification of the parents of either child b. If the probe used in question‘flleads to the conclusion that a child can not belong to a particular set of parents, the new probe can reverse the conclusion N “T7 @a and b apply ; . The question can not be answered {5‘ SJ. The sequence cleaved by the enzyme Ha—III is: --- G _CC-—~ cc GG--- Using a probe for a portion of the sequence of the gene foi'fii/lzherase you obtain the following result in normal and mutant lives cells, after cleavage of DNA, electmphoresis and assay with a probe for this region of DNA and find: Normal Mutant base pairs 103 lycine — Arginine mei-a .‘ c. Proline - Threonine' 2nd base in ca don 18! base In codon uopoo ul oszq p.19 ...
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Test 1 Spring 2006 - / ". @ ejO’lJmmS > f "f...

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