Approach Lab 10

Approach Lab 10 - Once we had the amplified DNA we...

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For this lab, we wanted to learn about genetically modified organisms (GMO) and their reason for existing. To do this, we wanted to learn techniques like polymerase chain reaction (PCR) and gel electrophoresis, and use these to determine if a particular food product contains soy or not. The polymerase chain reaction works by amplifying a few pieces of DNA, generating millions of copies of DNA. This allowed us examine the DNA for genes that are contained in soy, such as lectin. When performing polymerase chain reaction, we add a primer which serves to help amplify the genes. The product we are amplifying is expected to be 226 base pairs in length.
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Unformatted text preview: Once we had the amplified DNA, we performed gel electrophoresis. To perform gel electrophoresis, we first had to make our own 2.5% agarose gel and then dump it into our gel electrophoresis container, which has a power supply running through it. Once we did this, we had to wait 10 minutes for the gel to set. Then we loaded our polymerase chain reactioned samples into separate lanes. Once they were all in, we ran the gel by setting the power supply to a voltage of 100 volts. To visualize the gel, we used the gel doc and printed a picture. From here, we could tell if our food contained soy products or not....
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