Approach to lab 5

Approach to lab 5 - Approach: We used subcellular...

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Approach: We used subcellular fractionation to separate distinct parts of intracellular compartments. This allows us to examine the properties of the organelles individually. To do this, we had to disrupt the cells which allowed us to separate the organelles by centrifugation. The subcellular components were isolated differently based on their size and density because different sizes and densities cause each organelle to behave differently to the centrifuge. We could then examine each part under a microscope. Once we had examined the subcellular fractions under a microscope, we could analyze the chloroplast fraction by measuring enzymatic activity in photosynthesis because photosynthesis occurs in the chloroplast, so we would see how much the enzyme speeds up photosynthesis. The process used is called the Hill reaction, which is the transfer of electrons to an artificial electron acceptor. Water is split in photosynthesis to Photosystem 2, on to Photosystem 1, and eventually to NADP+, but the artificial electron
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This note was uploaded on 10/25/2011 for the course ENGR 110 taught by Professor Cardinal during the Spring '08 term at Cal Poly.

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