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Duong_Richard_Prelab12

Duong_Richard_Prelab12 - Richard Duong MCRO 251 Lab 30...

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Richard Duong MCRO 251 Lab 30 November 2010 Week 12 Pre-Lab Purpose: The objective of this lab is to examine and test how Enzyme-Linked Immunosorbent Assays (ELISAs) work on antigens and antibodies. Also, in this experiment we will observe the agglutination of large particulate antigens dealing with ABO blood groups (erythrocytes) and also the agglutination of smaller antigens such as Salmonella bacteria. Procedure: ELISAs 1. Recorde the number on your patient serum tube. 2. Retrieve microtiter strip that was filled with 2% antigen solution done two weeks ago and with a shake any unbound antigen solution into the sink. 3. Rinse very gently, flipping the strip of wells again afterward making sure that the wells fill up with water each time. Repeat this step twice. 4. Use a piPump and 1.0mL serological pipet to put 0.6mL of Tris buffer into each of these five empty tubes. 5. Use flick technique to briefly mix tube of ‘patient serum’ and then label the five 13 x 100 mm tubes of Tris buffer as follows: 1:4, 1:16, 1:64, 1:256, and 1:1024. 6. Use a micropipete to draw up 0.2mL of mixed contents of primary antibody and transfer to the 1:4 tube. Discard this micropipet into BacDown beaker.
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