M101A_2011Mid1KEY.Corrected - MCDB 101A M2011 Midterm1...

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1 MCDB 101A M2011 Midterm1 Name______ Key__ _________________________ 10/18/11 (100 points total) Perm #____________________________________ Multiple Choice: Circle the letter corresponding to the best answer (3 points for each correct answer) 1. Which of the following is a restriction enzyme recognition sequence? a. 5' GGTACC 3' b. 5' AATTAA 3' c. 5' AATGCATT 3' d. a and c e. a, b, c 2. Franklin’s X-ray diffraction experiments provided direct information about which of these features? a. The number of hydrogen bonds that link the bases together b. The average length of a DNA molecule c. The length of a DNA helix turn d. The antiparallel orientation of the two DNA strands e. Both a and b 3. Which feature is critical for the proof-reading ability of DNA polymerases? a. 5’ to 3’ exonuclease activity b. 3’ to 5’ exonuclease activity c. Their alpha subunits d. Their beta subunits e. Both c and d 4. Restriction endonucleases and topoisomerase II share which of these features in common? a. They cut both strands of a double-stranded DNA molecule b. They are able to rejoin the ends of a broken double-stranded DNA molecule c. They can alter the number of supercoils in a closed circular DNA molecule d. They create single-stranded nicks in a DNA molecule while leaving the opposite strand intact e. both a and c 5. Cesium chloride density gradient centrifugation was used in which of the following? a. The Hershey-Chase experiment b. The Griffith experiment c. The Meselson-Stahl experiment d. Determination of the genetic code e. The Beadle and Tatum experiment 6. To do a cis test you coinfect E. coli B with two rII phage. You make two ten-fold dilutions of the lysate, add 100 ml of this solution to E. coli B and spread the mixture on a plate of solid media. 100 plaques appear on the plate. Prior to the dilutions, how many phage per ml were present in the bacterial lysate? a. 1 X 10 5 b. 100 c. 10 d. 1 X 10 3 e. none of the above
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2 7. The large cloudy plaques that are seen when T2 phage are added to a mixture of E. coli B and B/2 are characteristic of which of the following genotypes? a. HR b. Hr c. hR d. hr e. Hh and Rr 8. Which of these proteins is required for rolling circle replication? a. DNA A protein b. DNA helicase c. Primase d. DNA gyrase e. all of the above 9. True or false : According to Chargaff’s rules, A+T = G+C. (3 points) 10. Name the three functional classes of proteins associated with auxotrophic mutations. (6 points) Enzymes, transport molecules, and regulatory proteins 11. Watson and Crick’s DNA structure differed in two significant ways from those proposed by other
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This note was uploaded on 10/27/2011 for the course MCDB 101A taught by Professor Thrower during the Fall '08 term at UCSB.

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M101A_2011Mid1KEY.Corrected - MCDB 101A M2011 Midterm1...

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