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Unformatted text preview: MCB 3020 Test 2 Spring 01 Name__________________________ 1. One hundred bacteria multiply into 10,000,000 bacteria in 100 minutes. What is the generation time? a. 10 min b. 0.1 hr c. 0.6 hr d. 0.2 hr e. 20 minutes 2. Eighty bacteria per mL contaminate a donut. The volume of the donut is approximately 7mL. The generation time is 20 minutes. After 8 hours, what was the concentration of bacteria i.e number per mL? a. 80 x 10 x 2 8 b. 80 x 10 24 c. 80 x 2 24 d. 2 x 20 24 e. 20 x 2 24 x 7 ab. 80 x 2 20 ac. 80 x 2 8 ad. 80 x 2 24 x 7 3. Beginning with a bacterial culture containing 1 organisms per mL, how long would it be before there were 1,000,000 organisms per mL, assuming a generation time of 30 minutes. a. 250 min b. 1550 min c. 600 min d. 700 min e. 1300 min 4. What is n (the number of generations) when 10 5 cells multiply into 10 11 in 12 hours. a. (log 10 5 log 10 11 )/ 0.301 b. (log 10 11 log 10 5 ) / .301 c. 0.301 / 115 d. 0.301 (12hr) / (11 5) e. .301 (115) / 12 5. Which of previous question’s answers is the generation time g? (Use # 4 answers) 6. Calculate the growth rate constant k, if the generation time is 10 min.?(Careful with hr & min.) a. 10 x n doublings per minute b. 1/10 doublings per hour c. 4 doublings per hour d. 6 doublings per hour e. 2 doublings per hour 7. Six cells multiply into 96 cells in 60 minutes. What is the generation time? a. 20 minutes b. 60 minutes c. 30 minutes d. .301(90 min) / log 96 e. 15 min 8. Which of the following does not contain a high energy bond? 9. What method is used to produce only surface colonies in the viable plate count method? a. spread plate b. block plate c. streak plate d. pour plate e. a, c 10. The redox potential of the redox pair that accepts electrons is .20 and that of the redox pair losing electrons is .8. If 4 equivalents of electrons are transferred, what is the free energy change? Write answer with correct signs in right places. a. (4)(23)(0.6) kcal b. (4)(46)(0.6) calories c. (4)(23)(1.0) kcal d. (4)(23)(1.0) calories e. (4)(23)(0.6)kcal 11. Choose the best energy source? a. H 2 b. SO 42 c. NO 2 d. Fe +3 e. b,c 12. For the reaction, monosaccharide + monosaccharide disaccharide, a. ^G is negative b. reaction procedes if the subunits are first activated and then water is removed c. the amino acid subunits spontaneously are linked together into the dipeptide d. the reaction is an example of phase III or assembly e. c,d 13. The water activity a. is the ratio of the vapor pressure of pure water to the vapor pressure above the solution b. that is needed for fungi is .995 c. decreases as the solute concentration increases d. is the negative log {H +...
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This note was uploaded on 10/29/2011 for the course MCB 3020 taught by Professor White during the Spring '09 term at University of Central Florida.
 Spring '09
 WHITE
 Bacteria

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