BIOCHEM LAB 6.docx - Lab 6 Pathway building Pentose phosphate pathway Theory based lab focusing on key mechanisms of amino acid generation and bridging

BIOCHEM LAB 6.docx - Lab 6 Pathway building Pentose...

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Lab 6: Pathway building - Pentose phosphate pathway Theory based lab focusing on key mechanisms of amino acid generation and bridging them to the citric acid cycle and glycolysis pathway (dry) Lab Objectives: 1. Examine the Pentose Phosphate Pathway 2. Diagram the processes of fatty acid degradation Part 1 - Designing your worksheets From last weeks lab, you should have the beginnings of a metabolic map. Your map should look something like the following, hopefully with more structures, enzymes and elaborated sugar pathways filled in. Today we are building on building on this map, by adding in components of the pentose phosphate pathway and fatty acid degradation. This will be achieved utilizing pages 1 and 3 1 CHEM-330
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Combined with the parts of the poster obtained last week (shown in grey), you should have the following amount of the poster filled in. As a reminder, red boxes will indicate structures, black boxes will indicate enzymes. Green boxes indicate major metabolites that you will need to connect to other pathways (more on this later) and purple boxes represents the end of a pathway. Blue boxes indicate an area where you will 2 CHEM-330
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have to figure out how the pathway comes together. You will need to fill in both structures and enzymes in blue boxes To begin, we will fill in the following diagram with steps learned from this week's worksheet Question 1: (10 points) Fill in boxes labelled Q1 on worksheets 1 and 3 with its corresponding structures and enzymes. Red boxes indicate structures, black boxes indicate enzymes.
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