308 Questions 5-7

308 Questions 5-7 - How does complexity of bacterial genome...

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How does complexity of bacterial genome differ from that of eukaryotic (for example calf) genome? Bacterial genomes only contain unique sequences and thus no repetitive sequences Calf genomes have 3 regions – highly repetitive, moderately repetitive, and unique sequences Explain C value paradox. States there is no correlation between the size of the genome and the complexity of an organism List and briefly explain factors that influence DNA renaturation kinetics. Complexity – simple sequences will renature faster Ionic strength – salt concentration can mask the repulsion forces of the negative phosphate backbone Low temperature Size of fragment Reaction time You have found a new species of insects. To evaluate the complexity of the genome of this species, you isolate genomic DNA from, fragment the DNA to uniform 500 base pair pieces, denature the DNA and measure the rate of reassociation. Your data is represented in the curve below (sorry for the bad drawing): How many classes of DNA (in respect to sequence complexity ) are found in this organism? 3 classes of DNA are found o Highly repetitive o Moderately repetitive o Single copies that are unique What can you say about the relative complexity of each class? What fraction of the genome (in %) falls into each class? 45-50% are single copy, 25% are moderately repetitive, and 25-30% are highly repetitive
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308 Questions 5-7 - How does complexity of bacterial genome...

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