308 Questions 16-19

308 Questions 16-19 - 1 You have discovered base changes in...

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1. You have discovered base changes in the promoter region of the operon in a bacterial chromosome. Would you expect these changes to act in trans on another copy of the operon? Explain your reasoning. 2. What are cis- elements? What are trans- factors? Give an example from the Trp operon (or form the Ara-operon). 3. Draw the diagram of the lac operon that illustrates negative control (be careful here and think about the complete picture !!!!!). 4. You have isolated a protein that binds to DNA in the region upstream of the promoter sequence of the gene of interest. If this is a positive regulator (activator) which would be true: A) Loss of function mutation in the gene encoding this DNA binding protein would cause constitutive expression B) Loss of function mutation in the gene encoding this DNA binding protein would result in lower or no expression. Explain your reasoning. 5. Discuss why are lac O c mutants cis-acting. 6.
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308 Questions 16-19 - 1 You have discovered base changes in...

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