Topic 12 - Topic 12 Microbial Genomics genomics: the...

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Topic 12 Microbial Genomics
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genomics: the discipline involving mapping, sequencing, analyzing and comparing genomes functional genomics: assigning function to unknown genes by analyzing biochemical and physiological effects of mutants transcriptome (transcriptomics): complement of mRNAs produced in an organism under a specific set of conditions proteome (proteomics): total complement of proteins present in a cell, tissue, or organism at any one time
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metabolome (metabolomics) the complete set of metabolic intermediates and other small molecules produced in an organism bioinformatics use of computational tools to analyze, compare, store, & access DNA sequences metagenomics characterization of DNA obtained directly from environmental samples
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G e n o m i c s
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Genomics Genome entire complement of genetic information includes genes, regulatory sequences, non-coding sequences Genomics process of mapping, sequencing, analyzing and comparing genomes
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History of Genomics 1976: first complete genome sequenced MS2 (RNA virus) 3.53 kbp 1995: first cellular genome sequenced Haemophilus influenzae 1.83 Mbp Currently, >2500 prokaryotic genomes sequenced or in progress
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This technique has three steps: Step 1 - Original DNA is broken into a collection of fragments. Step 2 – DNA cloned into plasmids and each “insert” sequenced (shotgun sequencing). Step 3 – Assemble “reads” with dedicated molecular biology software. Genome sequencing http://tinyurl.com/DNAbaser
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ORF: sequence of DNA that, if transcribed, could be translated to yield a protein of known length, composition open reading frames (ORFs) i.e. annotation
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size (Mb) locations of tRNA genes rRNA operon IS, other repetitive DNA elements histogram showing regional G+C content th ed Chromosome map ( Mycobacterium tuberculosis ) ORFs
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Topic 12 - Topic 12 Microbial Genomics genomics: the...

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