140 - Topic 6 Notes

In nature water activity is commonly influenced

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Unformatted text preview: ry good oxidizers (i.e. causes other compounds to oxidize). Organisms that are able to grow in the presence of O2 are usually able to detoxify these byproducts (Figure 5.29) using specific enzymes such as catalase, peroxidase and superoxide dismutase. Since O2 is not very soluble in water, to culture aerobic microorganisms, it is necessary to aerate the culture by either shaking the tube or flask or by bubbling air into the culture medium. In contrast, growth of obligate anaerobes requires that O2 be excluded from the culture medium. Thioglycolate broth, along with the redox indicator dye resazurin, can be used as a test to demonstrate the aerobic or anaerobic growth characteristics of a given microorganism (Figure 5.26)....
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