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BIO111 – General College Biology I with Lab – Photosynthesis Lab ActivityPage 1of 8BIO111Photosynthesis Lab ActivityPurpose:In this experiment we will determine if the plant disks will float without the presence of light in a Sodium Bicarbonate and water mixture. Two different plant leaves will be tested. 10 spinach disks from leaves and 10 Thai basil disks form leaves. Hypothesis:In this experiment, The hypothesis will be test and confirm that without the presence of light, no disk leaves (Thai Basil or Spinach) will float from the bottomto the surface in a Sodium Bicarbonate and water mixture.Materials:Baking sodaMeasuring teaspoonDistilled waterRuler Disk soapHealthy fresh Spinach and Thai basil leavesSeveral clear plastic cupsHand hole punch 1 plastic syringe (10 cc or larger) counter space with minimal light. SpoonRuler Methods (Procedures):
BIO111 – General College Biology I with Lab – Photosynthesis Lab ActivityPage 2of 81.Prepare the bicarbonate solution used in this trial. Take 300 ml of distilledwater and place approximately 1/8 of a teaspoon in the solution. This solution is approximately .2% solution of carbon dioxide and serves as analternate source of carbon dioxide. 2.Take the spinach and the Thai Basil leaves, make 20 circles of each using the hole puncture. Be careful to avoid the veins and the thicker areas of the leaves.3.Prepare 4 small containers to 2cm line measured by ruler and marked. 2 of these small containers are controls of distilled water and a drop of soap. Label the controls as follows: control spinach and control Thai Basil. The other two small containers are sodium bicarbonate mixture with on the one drop of soap. Fill to the 2 cm line and label as follows: spinach Sodium Bicarbonate and Thai Basil Bicarbonate.