DNA_Electro

DNA_Electro - 1 DNA ELECTROPHORESIS The human body is made...

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Unformatted text preview: 1 DNA / ELECTROPHORESIS The human body is made up of approximately 60 trillion cells which all work together. In Eukaryotic organisms (like humans) the genetic material (DNA) is enclosed within a nucleus. DNA - deoxyribonucleic acid; contains all necessary information to direct cell reproduction and other processes Mitosis Review The sequence of bases in DNA represents the “Genetic Code”. With the exception of identical twins, each persons DNA sequence is unique. Special enzymes (proteins) called “Restriction Enzymes” cut DNA into fragments. An Individual can be positively )$%.4)=%$ "9 ! ;=.'%202).4< of these fragments. This ;¡¢£ =.'%202).4< #!. "% visualized through a technique called Gel Electrophoresis. ¡¢£ ¤=.'%202).4¤ -!$% "9 #544).' a DNA sample into fragments DNA is negatively charged (-). The negative charge allows for separation and visualization by Gel Electrophoresis. Gel Electrophoresis / DNA Fingerprinting @ DNA is negatively charged @ Gel made from agarose @ DNA separated according to size @ Electric current repels / attracts mixture of DNA fragments through microscopic pores in the gel from the negative to the positive electrode @ Shortest, smallest fragments move fastest and move to the bottom of the gel @ Longer, slower fragments remain at the top of the gel @ DNA can be visualized by staining with an appropriate dye 2 Applications of DNA Fingerprinting @ forensic science @ paternity testing @ genetic testing @ evolutionary studies @ others Loading DNA Samples Loading DNA Samples Which suspect is most likely guilty?...
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This document was uploaded on 11/01/2011 for the course BSC bsc1005l at FSU.

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DNA_Electro - 1 DNA ELECTROPHORESIS The human body is made...

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