bt 117 lab Safety in the Cell Culture Lab©2009

bt 117 lab Safety in the Cell Culture Lab©2009...

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Safety in the Cell Culture Lab©2009 Dr. Collins Jones Biotechnology Program Montgomery College Copyright 2009 all rights reserved. This material may not be reproduced in any form.
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In this presentation you will learn Basic Safety Rules Types of Safety Hazards in the cell culture laboratory Biohazards and Biosafety Levels Simple Testing for Biohazards
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Basic Types of Laboratory Hazards Chemical Biological Temperature Electrical Mechanical Radiation
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Please note: We do not use bio-hazardous cell lines in BT 117 Both Sf9 and CHO cells have GRAS status We do not use strong acid, base, oxidizers or mutagenic chemicals We do not use glassware, needles or other sharp objects It will take an effort on your part to cause an injury to yourself in the BT 117 lab!
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Safety Rules for Any Lab– Your Responsibility No eating or drinking in the lab Nothing goes in your mouth in the lab - ever Food or drink cannot enter the lab even if it will not be consumed Any food or drink found in the lab will be confiscated and thrown out – no exceptions! Do not apply cosmetics (make-up) in the lab Keep your fingers / hands away from your face including your hair and mouth Wear eye protection at all times
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Your Responsibilities While in the lab you must wear appropriate PPE: Dress yourself - lab coat and gloves Protect your eyes – safety glasses Control your hair – use a hair cover and tie it back, don’t let it become part of the experiment! No stinky feet – your entire foot must be enclosed inside a shoe – no sandals or clogs Wash your hands on entry and exit from the lab
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PPE: Eye Protection - Meet BOB Appropriate eye protection consists of one of the following safety glasses goggles a face shield Cover your eyes!
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Inappropriate Eye Protection Be a good Bob! Wear appropriate eye protection over your eyes!
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Control Your Hair Long loose hair is a hazard It can dip into chemicals or media causing contamination It can catch fire, be caught in equipment, obscure vision
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PPE –Hair Management Control your hair! Controlled Controlled Controlled Tie or clamp it, cut it or cover it – but control your hair!
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PPE – Foot Wear No stinky feet! Cover your feet
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Personal protective measures include Hand washing at start and finish of work and as needed during work Do not touch, taste, smell or otherwise expose yourself to hazardous material Proper training Proper PPE – check gloves frequently for holes or
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This document was uploaded on 11/03/2011 for the course BT 117 at Montgomery College.

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bt 117 lab Safety in the Cell Culture Lab©2009...

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