BT 117 Lab SOPs Sf9 Cells 2010

BT 117 Lab SOPs Sf9 Cells 2010 - Dr. Collins Jones...

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Unformatted text preview: Dr. Collins Jones Biotechnology Program Room 421 HTSC Montgomery College 240-567-1910 BT 117 Cell Culture and Cell Funtion Germantown, MD [email protected] Feeding and Passage of Sf9 Cells©2010 BSMaT Procedures for the Growth and Maintenance of Sf9 Cells©2010 BT-117 Cell Culture and Cell Function Biotechnology Program Montgomery College Germantown MD Prepared by: Dr. Collins Jones Biotechnology Coordinator Room 421 HT [email protected] 240-567-1910 Copyright 2010. All rights reserved. This document may not be reproduced, stored in or introduced into a retrieval system or transmitted, in any form or by any means (such as photocopying, scanning, recording,..) in part or in whole without the written permission of the author.- page 1 of 72 - Ver. 9/19/10 Dr. Collins Jones Biotechnology Program Room 421 HTSC Montgomery College 240-567-1910 BT 117 Cell Culture and Cell Funtion Germantown, MD [email protected] Feeding and Passage of Sf9 Cells©2010 BSMaT Contents for Sf9 Procedures ____________________________________________________________________________ Protocol Title Designation Page ____________________________________________________________________________ Retrieval, Thawing and Seeding of Sf9 Cells BT117.SF9001 from a Working Cell Bank ____________________________________________________________________________ Passaging of Sf9 Cells using a Cell BT117.SF9002 Scraper for Detachment ____________________________________________________________________________ Use of Cell Stripper™ to Detach Sf9 Cells BT117.SF9003 ____________________________________________________________________________ Feeding of Sf9 Cells Grown as BT117.SF9004 Anchorage Dependent Cells ____________________________________________________________________________ Cryopreservation of SF9 Cells BT117.SF9005 ____________________________________________________________________________ Recovery of SF9 Cells Grown as BT117.SF9006 Anchorage Dependent Cells in Large Volumes of Media ____________________________________________________________________________ Scale-up of Sf9 Cells for 500 ml or BT117.SF9007 not available 1.0 liter Suspension Cultures ____________________________________________________________________________ Growth of Sf9 Cells in Roller Bottles BT117.SF9008 not available ___________________________________________________________________________ General Checklist for Subculture Preparation- ____________________________________________________________________________ Incubator Organization Scheme- ____________________________________________________________________________ Tissue Culture Growth Flasks and Plates -- Recommended Volumes ____________________________________________________________________________- page 2 of 72 - Ver. 9/19/10 Dr. Collins Jones Biotechnology Program Room 421 HTSC Montgomery College 240-567-1910 BT 117 Cell Culture and Cell Funtion Germantown, MD [email protected] Feeding and Passage of Sf9 Cells©2010...
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This document was uploaded on 11/03/2011 for the course BT 117 at Montgomery College.

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BT 117 Lab SOPs Sf9 Cells 2010 - Dr. Collins Jones...

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