05 - Take Home Quiz 5 W11 - Chromatin VNTR

05 - Take Home Quiz 5 W11 - Chromatin VNTR - Take Home Quiz...

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Take Home Quiz 5 Due at the beginning of class Friday February 4, 2011 Name_____________________________________ 1. [6 points] From a mixture, you are separating two proteins using polyacrylamide gel electrophoresis. The proteins are 30,000 MW and 60,000 MW. The proteins were boiled in the presence of SDS prior to loading in the gel. Diagram the gel after electrophoresis and staining with Coomasie blue. Correctly diagram the following: gel, relative locations of the 30,000 MW and 60,000 MW proteins, well, positive charge electrode, and negative charge electrode. 2. [2 points] Which of the following ligands (Circle one) would you use to isolate histones using affinity chromatography? ENZYME RNA DNA HORMONE CELL 3. [2 points] Explain your answer to Question 2 in one sentence. 4. [4 points] Figure 1 shows the DNA fingerprints of five people: a child, mother and three potential fathers. Which males can be ruled out as being the father?
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This document was uploaded on 11/04/2011 for the course CHEM 106 at BYU.

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05 - Take Home Quiz 5 W11 - Chromatin VNTR - Take Home Quiz...

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