Restriction mapping homework

Restriction mapping homework - Restriction mapping homework...

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Unformatted text preview: Restriction mapping homework Group Homework Assignment #1 20 points possible How to receive credit for completing the assignment Write up your answers to the 4 questions labeled in orange on the next slide Just hand-write on paper Include at the top the names of other people you worked with on this assignment (group work is encouraged, but not mandatory) Only turn in one assignment with your name on it!! Turn in to TAs at the beginning of class on Friday Sept. 23rd Group homework exercise #1 You have a plasmid (circular DNA!!) that is 10,100bp in size and you cut it with the PstI restriction enzyme, then run out on a gel Resulting DNA fragments are 1,900 + 3,100 + 5,100 (try to construct a restriction map based on this info. Question #1: What do you find? Are you able assemble a map in the correct order ? ) You cut the same plasmid with HindIII restriction enzyme Resulting DNA fragments are 2,500 + 7,600 (try to construct a map using this information in conjunction with the above information. Question #2: What do you find? Are you able assemble a map in the correct order ? ) You cut the same plasmid with both PstI and HindIII enzymes at the same time (a double digest) Resulting DNA fragments are 500 + 1,100 + 1,400 + 3,100 + 4,000...
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Restriction mapping homework - Restriction mapping homework...

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