Enzymes and Isolated Reaction1

Enzymes and Isolated Reaction1 - Enzymes and Isolated...

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Enzymes and Isolated Reactions: Activities of FA Synthase The first iteration of the sequence catalyzed by this enzyme can be represented by the seven following reactions. 1. In the first reaction acetyl CoA is added to a cysteine -SH group of the condensing enzyme (CE) domain: acetyl CoA + CE-cys-SH -> acetyl-cys-CE + CoASH Mechanistically this is a two step process, in which the group is first transferred to the ACP (acyl carrier peptide), and then to the cysteine -SH group of the condensing enzyme domain. 2. In the second reaction malonyl CoA is added to the ACP sulfhydryl group: malonyl CoA + ACP-SH -> malonyl ACP + CoASH This -SH group is part of a phosphopantethenic acid prosthetic group of the ACP. 3. In the third reaction the acetyl group is transferred to the malonyl group with the release of carbon dioxide: malonyl ACP + acetyl-cys-CE -> beta-ketobutyryl-ACP + CO 2 4. In the fourth reaction the keto group is reduced to a hydroxyl group by the beta-ketoacyl reductase activity:
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This document was uploaded on 11/03/2011 for the course BIOLOGY BSC1105 at Broward College.

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Enzymes and Isolated Reaction1 - Enzymes and Isolated...

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