Experiment_2__Glucose_Lab_Report Experiment_2

Experiment_2__Glucose_Lab_Report Experiment_2 - EXPERIMENT...

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EXPERIMENT 2: BIOANALYTICAL ANALYSIS OF GLUCOSE (INFORMAL) Data and Results ____22___ / 24 pts Table of absorbance-concentration data. (5 points) 10 Table of glucose concentration data for all the samples. (5 points) Calculations were done correctly. (4 points) 4 Plot of Absorbance vs. Concentration with all the data points (6 points) 8 Calibration plot of linear region only with linear regression properly applied. (4 points) To receive full credit the plots must be properly titled and its axis properly labeled, with proper units . Sample Calculations ____6___/ 6 pts Show the calculation used to determine the concentration of glucose in the original, undiluted sample of one of the beverages. The sample calculation should show how the dilutions were taken into account to get back to the original concentration. 8 Questions: _38____ / 54 pts Question 1: 2 points
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Question 2: 6 points Question 3: 8 points Question 4: 8 points Question 5: 8 points Question 6: 8 points Question 7: 8 points Question 8: 6 points Conclusion ___8__ / 8 pts Style (organization, spelling, neatness, grammar, etc…) __8___ / 8 pts Other Deductions (i.e., Late Lab Report) - _____ Score __90___ / 100 pts
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Experiment 2: Bioanalytical Analysis of Glucose in Sports Drinks Pledge
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Results and Calculations : In order to determine the amount of glucose in sports drinks, sugar solutions, and an unknown sample, seven solutions of varying amounts of glucose, phosphate buffer, Ferrocyanide, and enzyme mix were made to serve as standards in seven test tubes. The enzyme mix was prepared with glucose oxidase and horseradish peroxidase. An auto pipet was used to deliver the desired volume of each component into a test tube in the order seen in Table 1 below: Phosphate Buffer, Glucose Solution, Ferrocyanide, enzyme. Table 1 also shows the volume of each component added. The total volume in each test tube was 5 mL. Sample Number [Glucose] mM Volume Phosphate Buffer (mL) Volume 1mM Glucose Solution Volume of Ferrocyanide (mL) Volume of Enzyme Mix (mL) 1 0.00 3.5 0 1.0 0.5 2 0.10 3.0 0.5 1.0 0.5 3 0.20 2.5 1.0 1.0 0.5 4 0.30 2.0 1.5 1.0 0.5 5 0.40 1.5 2.0 1.0 0.5 6 0.50 1.0 2.5 1.0 0.5 7 0.60 0.5 3.0 1.0 0.5 Table 1: Summary of phosphate buffer, glucose solution, Ferrocyanide and Enzyme Mix in Sample Eight more solutions were made in eight separate test tubes. These real world samples were prepared the same way as the standard samples. Using an auto pipet, the desired volume of each component was delivered to a test tube in the order seen in Table 2 below, from left to right. The real world samples were used to analyze samples of 50x diluted Gatorade, Propel, an undigested sugar solution, and a digested sugar solution. These solutions were diluted beforehand in the prep room and each of the preparations further diluted the samples as described later. Additionally, there was one sample of an unknown solution. The unknown solution number was 8. In each of the test tubes, ferrocyanide was added, followed by phosphate
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This document was uploaded on 11/04/2011 for the course CHEM 241 at UNC.

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Experiment_2__Glucose_Lab_Report Experiment_2 - EXPERIMENT...

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