Experiment_5__GPC_Lab_Report_rduong_duongExperiment5

Experiment_5__GPC_Lab_Report_rduong_duongExperiment5 -...

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EXPERIMENT 5: GEL PERMEATION CHROMATOGRAPHY (INFORMAL) Data: __14___ / 16 pts • Table of elution volumes obtained for the four samples (Dextran Blue, Cytochrome c, Phenol Red, Hemoglobin). (10 points) • Table containing experimental molecular weight and literature molecular weight of Hemoglobin. (6 points) Note: The literature value must come from a primary literature source. Sample Calculations: ___6__ / 6 pts Calculation of apparent molecular weight of Hemoglobin Results: __19___ / 21 pts Plot of Log Molecular Weight vs. Retention Volume (V r ). (15 points) Determination of the apparent MW of hemoglobin (6 points) 7 Questions: ___29__ / 37 pts Question 1: 6 points Question 2: 5 points Question 3: 5 points Question 4: 5 points Question 5: 5 points Question 6: 5 points
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Question 7: 6 points Conclusion: __10___ / 10 pts Style (organization, spelling, neatness, grammar, etc…) ___10__ / 10 pts Other Deductions (i.e., Late Lab Report, incorrect formatting, etc…) - _____ Score __88___ / 100 pts Experiment 5: The Determination of the Molecular Weight of Hemoglobin by Gel Permeation Chromatography Richard Duong Lab Partner: Britt Mansfield October 29, 2009 Christopher Backlund Chem 241L, Section 420, Chapman Hall 125
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The work presented in this report is my own, and the data was obtained by my lab partner and me during the lab period. Results and Calculations Table of retention volume compared to samples’ molecular weight: Retention Volume (mL) Molecular Weight (Da) Log Molecular Weight Dextran Blue 2000 9.00 2 x 10 6 4.90 Cytochrome c 16.00 12327 4.08 Phenol Red 43.00 354.38 3.48 Hemoglobin
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This document was uploaded on 11/04/2011 for the course CHEM 241 at UNC.

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Experiment_5__GPC_Lab_Report_rduong_duongExperiment5 -...

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