Experiment_6__LC_Lab_Report_Experiment_6

Experiment_6__LC_Lab_Report_Experiment_6 - EXPERIMENT 6:...

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EXPERIMENT 6: LIQUID CHROMATOGRAPHY (FORMAL) Introduction __9____ / 10 pts Procedure ___5___ / 5 pts Data and Results __22____/ 22 pts Properly labeled chromatogram of spinach extract. (6 points) Data table containing pigment name, observed color, and retention times from the chromatographic separation. (6 points) Table of R and α results. (5 points) Red cabbage extract acid-base results and observations. (5 points) Sample Calculations __10____ / 10 pts Relative Retenition (α) (5 points) Resolution (5 points) Discussion __11____ / 12 pts Extraction and chromatography results. Discuss the observations you made upon adding the various drops of 0.1 M NaOH and 0.1 M HCl to the red cabbage extract. Chemically, discuss why changing the pH had an affect on the red cabbage extract. Problems and errors during the experiment. Observations and conclusions. 9 Questions (4 points each) __33____ / 36 pts Style (organization, spelling, neatness, grammar, etc…) ___5__ / 5 pts Other Deductions (i.e., Late Lab Report, incorrect formatting, etc…) - _____ Lab Report Score __95____ / 100 pts
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Experiment 6: The Extraction of Plant Pigments from Spinach and the Subsequent Separation and Analysis of the Extracted Pigments Using Liquid Chromatography Pledge Introduction: The objective of this experiment is to use the solvent-solvent extraction method with reversed-phase liquid chromatography and spectrophotometric detection to separate and analyze extracted pigments from spinach 1 . In a second part of the experiment the effects of pH of plant pigments will be investigated by adding drops of 0.1M NaOH or 0.1M HCl to 1mL cabbage extracts. This type of experiment is important when trying to distinguish components of
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complex mixtures. Plant pigments can be classified into three major groups: porphyrins, carotenoids, and flavenoids. Porphyrins include chlorophyll a, which displays a green color, and chlorophyll b, which exhibits a yellow-green color 1 . The carotenoids include the carotenes and xanthophylls 1 . Examples of carotenes are α and β carotenes (yellow) 1 . The xanthophylls include lutein, zeaxanthin, violaxanthin, and neoxanthin (yellow). The last group are the flavenoids, which include flavone (yellow), flavonol (yellow), and anthocyanin (red, blue, purple) 1 . In order to separate and analyze the pigments, there are three steps: sample preparation, chromatographic separation, and spectrophotometric detection. First, plant cells containing the pigments must be broken apart by grinding spinach in a blender with acetone and incubated and filtered. This is used to remove the pigments from the plant cells into an organic mixture. NaCl is used to make a biphasic mixture with flavonoids and trihydroxy Xanthophylls in the organic 1 . The organic phase will be further analyzed in the experiment so it is isolated.
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Experiment_6__LC_Lab_Report_Experiment_6 - EXPERIMENT 6:...

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