Experiment_6__LC_Lab_Report_rduong_duongExperiment6

Experiment_6__LC_Lab_Report_rduong_duongExperiment6 -...

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EXPERIMENT 6: LIQUID CHROMATOGRAPHY (FORMAL) Introduction ___6___ / 10 pts Procedure __4____ / 5 pts Data and Results ___18___/ 22 pts Properly labeled chromatogram of spinach extract. (6 points) Data table containing pigment name, observed color, and retention times from the chromatographic separation. (6 points) Table of R and α results. (5 points) Red cabbage extract acid-base results and observations. (5 points) Sample Calculations ___10___ / 10 pts Relative Retenition (α) (5 points) Resolution (5 points) Discussion __9____ / 12 pts Extraction and chromatography results. Discuss the observations you made upon adding the various drops of 0.1 M NaOH and 0.1 M HCl to the red cabbage extract. Chemically, discuss why changing the pH had an affect on the red cabbage extract.
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Problems and errors during the experiment. Observations and conclusions. 9 Questions (4 points each) ___33___ / 36 pts Style (organization, spelling, neatness, grammar, etc…) ___5__ / 5 pts Other Deductions (i.e., Late Lab Report, incorrect formatting, etc…) - _____ Lab Report Score ____85__ / 100 pts Experiment 6: The Extraction of Plant Pigments from Spinach and the Subsequent Separation and Analysis of the Extracted Pigments Using Liquid Chromatography Richard Duong Lab Partner: Britt Mansfield November 5, 2009 Christopher Backlund Chem 241L, Section 420, Chapman Hall 125
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The work presented in this report is my own, and the data was obtained by my lab partner and me during the lab period. Introduction In this experiment, the objective is to use solvent-solvent extractions to extract pigments from a spinach sample and then use the method of reversed-phase liquid chromatography to separate and analyze extracted pigments. The purpose of doing this experiment is to show that there are a plethora of chemicals within plant pigments that can be extracted by the solvent-solvent extraction method. This methodology is absolute-it is a very useful skill to have as an analytical chemist to be able to efficiently separate mixtures of different chemical makeup, and solvent- solvent extraction is one way to achieve this. Separating these different plant pigments and analyzing them separately, it can be seen each different pigment (porphyrins, carotenoids, and flavenoids) all have different characteristics in terms of color, function, and polarity. These different characteristics are all because they each have a different chemical makeup and make it possible to utilize solvent-solvent extraction based on polarity. The resulting extracted solution will then be chromatographically separated again, based on polarity. More specifically a reversed-phase, step-gradient liquid chromatography will be used; meaning, different mobile phases will be used, starting with a very non-polar phase to a less polar phase, in order to separate the extracted plant pigments. The solvents will be delivered by using a single piston pump. These different mobile phases used in this method, the step-gradient, is used because the
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This document was uploaded on 11/04/2011 for the course CHEM 241 at UNC.

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Experiment_6__LC_Lab_Report_rduong_duongExperiment6 -...

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