andrade6 - EXPERIMENT 6 LIQUID CHROMATOGRAPHY(FORMAL...

Info icon This preview shows pages 1–4. Sign up to view the full content.

View Full Document Right Arrow Icon
EXPERIMENT 6: LIQUID CHROMATOGRAPHY (FORMAL) Introduction ___9___ / 10 pts Procedure ___4___ / 5 pts Data and Results ___22___/ 22 pts Properly labeled chromatogram of spinach extract. (6 points) Data table containing pigment name, observed color, and retention times from the chromatographic separation. (6 points) Table of R and α results. (5 points) Red cabbage extract acid-base results and observations. (5 points) Sample Calculations ____8__ / 10 pts Relative Retention (α) (5 points) Resolution (5 points) Discussion ___12___ / 12 pts Extraction and chromatography results. Discuss the observations you made upon adding the various drops of 0.1 M NaOH and 0.1 M HCl to the red cabbage extract. Chemically, discuss why changing the pH had an affect on the red cabbage extract. Problems and errors during the experiment. Observations and conclusions. 9 Questions (4 points each) ____26__ / 36 pts Style (organization, spelling, neatness, grammar, etc…) __5___ / 5 pts Other Deductions (i.e., Late Lab Report, incorrect formatting, etc…) - _____ Lab Report Score ___86__ / 100 pts
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
The Extraction of Plant Pigments from Spinach and the Subsequent Separation and Analysis of the Extracted Pigments Using Liquid Chromatography Francisco Andrade Jessica Booe 5/12/10 Wesley Storm CHEM 241L 402, Morehead Labs Room 301 Pledge
Image of page 2
Introduction: The goal of this experiment is to extract the pigment from a prepared sample of spinach, and separate and analyze the pigments using reversed-phase liquid chromatography. A second part of the experiment that concerns the effects of pH on plant pigments will be investigated by adding 0.1M NaOH or NaCl to a prepared cabbage extract. These experiments will be carried out in order to learn about the process of liquid chromatography, and to learn about the pigments responsible for coloration in plants. The plan for studying the problem is to isolate the pigment molecules from spinach as much as possible, perform a liquid chromatographic extraction in order to separate the pigments from the rest of the organic matter in solution, and analyze the pigments and their distribution based on their polarity. The structure of plant pigments affects their polarity at the molecular level, and through reversed-phase liquid chromatography, one can analyze the pigments present in spinach. For example, chlorophyll a and chlorophyll b, two pigments which are responsible for the green coloration in plant leaves, are intermediate in polarity when compared with polar xanthophylls (yellow color) or carotenes (orange/red color) 1 . The spinach extract is a complex mixture suspended in a solvent, and by treating it with acetone, acetonitrile, and common table salt, one can isolate the organic compounds from the aqueous solution it is suspended in and create two phases: an upper organic phase containing the pigments and a lower aqueous phase.
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 4
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern