AP sites - uvrA, B and C ). The protein UvrA recognizes the...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
AP sites also form through spontaneous loss of a base. This occurs especially often with purines , for example several thousand times a day in a human cell. Repair of AP sites by AP endonuclease : cleaves adjacent to AP site. The deoxyribose is removed and the single base gap filled by DNA polymerase and ligase. Nucleotide excision repair removes a whole oligonucleotide that containes the damage. In E. coli three genes involved (
Background image of page 1
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: uvrA, B and C ). The protein UvrA recognizes the damage, recruits UvrB and UvrC which cleave at 3' and 5' site of damage, respectively, producing an oligonucleotide of 12 or 13 bases. Helicase necessary to remove oligonucleotide (disruption of hydrogen bonds from base pairing), DNA polymerase fills gap, ligase seals. Nucleotide excision repair also in eukaryotes . Similar mechanism....
View Full Document

Ask a homework question - tutors are online