Chemical Hydrolysis - If Nuclease hydrolyzes DNA they are...

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Chemical Hydrolysis At very low pH (1 or less) phosphodiester bond hydrolysis occurs accompanied by breakage of the N glycosylic bond between the base and the deoxyribose. At very high pH, DNA is resistant to hydrolysis. Enzymatic Hydrolysis Nucleases - enzymes which hydrolyze nucleic acids
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Unformatted text preview: If Nuclease hydrolyzes DNA they are termed DNAases DNAases are classified by the nature and location of their action ( Fig. 1 ): Single strand break Double strand break Cut only 3' or 5' end nucleotide from DNA ( exonuclease ) Cut only in middle of DNA ( endonuclease )...
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.

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