DNA - Similarities : 1) uses nucleoside 5'-triphosphates...

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DNA: the genetic material genetic information in the sequence of bases How can this information be used in the cell? First, the base sequence in DNA is used to synthesize RNA with complementary base sequence: Transcription Second, the base sequence in RNA is used to synthesize polypeptides with "complementary" amino acid sequence: Translation information flow from gene to protein, with base sequence in DNA determining amino acid sequence in protein. This concept is the: Central Dogma of Molecular Biology DNA-----transcription------> mRNA ------translation-----> protein Transcription in E. coli (in prokaryotes) : Enzyme involved in transcription- DNA dependent RNA polymerase RNA polymerase activity : some similarities, some differences to DNA polymerase activities
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Unformatted text preview: Similarities : 1) uses nucleoside 5'-triphosphates (NTPs) as precursors (Freifelder Fig. 9-1) 3) catalyzes phosphodiester bond between NTPs 4) uses DNA as template 7) base pairing determines correct base 8) growth of nucleic acid chain only in 5' to 3' direction 9) growing strand antiparallel to template strand Differences : 1) uses ribo nucleoside 5'-triphosphates instead of deoxyribonucleoside 5'-triphosphates (ATP, GTP, CTP, UTP) 2) can initiate the start of a new strand de novo (no primer necessary) (remember the RNA polymerase "primase" in replication) 3) a single strand of RNA is produced (only one strand of DNA used for RNA synthesis: the DNA template strand with complementary base sequence) 4) only short stretches of DNA are transcribed...
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