Enzymes Involved in Homologous Recombination

Enzymes Involved in Homologous Recombination - Enzymes...

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Enzymes Involved in Homologous Recombination Involved proteins were discovered through the analysis of recombination-defective mutants in E. coli Necessary proteins: 1) general enzymes connected with DNA biochemistry (DNA polymerases, ligase etc.) 2) several specific proteins. Central protein involved in homologous recombination is RecA Rec A promotes the exchange of strands between homologous DNAs that causes heteroduplexes to form. The action of RecA occurs in three stages (Fig. 5.34): 1) First RecA binds to single-stranded DNA, coating the DNA to form a protein-DNA filament. 2) RecA has two DNA binding sites and is able to bind to a second, double stranded DNA molecule, forming a complex between the two DNAs. This non- specific interaction (between protein and DNA) is followed by specific base pairing between the single-stranded DNA and its complement. 3) RecA catalyzes strand exchange, with the single strand originally coated with RecA displacing its homologous strand to form the heteroduplex. The
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.

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Enzymes Involved in Homologous Recombination - Enzymes...

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