Experiment - transcription factors have been identified 2...

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Experiment - Transcription of RNA Polymerase II In the test tube (in vitro) isolated RNA polymerase II plus DNA plus NTP ----- does not lead to transcription as was previously discovered for E. coli addition of other proteins -----successful transcription in vitro Conclusion: additional proteins must be present that fulfill role of the sigma subunit but are not primarily associated with RNA polymerase II Using in vitro experiments specific proteins involved in transcription (called
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Unformatted text preview: transcription factors) have been identified: 2 classes: basal transcription factors specific transcription factors Basal transcription factors necessary for transcription from all polymerase II promoters Specific transcription factors additionally necessary for transcription of a specific gene or a group of genes (specific regulation!)...
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