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Heteroduplex Analysis - single-celled eukaryotes or...

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Heteroduplex Analysis Renaturation studies can be combined with electron microscopy which allows you to actually visualize the DNA strands fragment #1: A-B-C-D-E-F-G-H A-B-C-D-E-F-G-H fragment #2: A-B-C-J-K-F-G-H A-B-C-J-K-F-G-H If you DENATURE, mix, then allow reannealing to occur between 1&2 it will look like the following heteroduplex ( Fig. 1 ): D-E A-B-C/ \F-G-H A-B-C\ /F-G-H J-K Classes of Renaturing Components 1 class of DNA fragments means that fragments are present in only 1 copy Repetitive sequences are common in multicellular eukaryotic organisms but not
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Unformatted text preview: single-celled eukaryotes or prokaryotes. 4 Classes of sequences in higher eukaryotes ( Fig. 4 ): unique [ 1 copy] (majority of gene sequences) slightly repetitive [ 1-10 copies] moderately repetitive [10-several 100 copies] highly repetitive [several hundred - several million copies] satellite DNA Sequences of normal length but which occur in very high #s of copies ex: genes which code for rRNAs (280 copies in humans) (450 copies in amphibians )...
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