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Impact of pH on Structure - Interestingly repulsive forces...

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Impact of pH on Structure pH changes can also be used to denature DNA Hydrogen bonding is very sensitive to H+ concentration At pH greater than 11.3 (alkaline conditions) all hydrogen bonds are eliminated and DNA is completely denatured (in single stranded form). Acid conditions also denature DNA but... there is damage to phosphodiester linkages. Phosphodiester bonds are resistant to alkaline pH so use of alkaline pH is method of choice for deliberately denaturing DNA in the laboratory. Impact of Salt on Structure
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Unformatted text preview: Interestingly - repulsive forces also are at play in the DNA double helix Recall the negatively charged phosphate groups. Charges are neutralized by bound + ions such as Na+ and Mg++. *In dH2O, repulsions are so great, strand sepration or denaturation occurs. Breathing Double stranded state is stable, but double stranded regions frequently open to become single-stranded bubbles. In AT rich areas , breathing is much more common. Denaturation is reversible...
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