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Unformatted text preview: as rolling circle replication . In this process, one strand of the F plasmid DNA is nicked at a specific sequence [the ori sequence] by a particular endonuclease encoded in the tra operon. Other proteins then bind to the 5' end of the nicked strand and guide that single strand (strand displacement) into the recipient cell (Figure). DNA polymerase can replicate a new daughter strand to replace the displaced strand. Once the displaced DNA strand crosses the conjugation tube and enters the recipient cell, a copy strand is replicated to yield double-stranded DNA which is finally converted into the usual circular and supercoiled form. In the end the original recipient cell is transformed into a donor cell and can now take part in spreading the plasmid....
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.
- Fall '10