Renaturation or Reannealing of DNA

Renaturation or Reannealing of DNA - enough so that the...

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Renaturation or Reannealing of DNA Very valuable technique which can be used to: 1) show genetic relatedness between organisms 2) can detect particular sequences (i.e. particular genes) if you have a DNA or RNA probe. 3) Can determine the frequency with which certain sequences appear [highly repetitive sequences will reanneal very rapidly]. Conditions for Renaturation Two conditions which must be met in order for Reannealing to occur: 1) 0.15m to 0.5M NaCl concentration ( the salt concentration must be high
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Unformatted text preview: enough so that the phosphate groups of the two DNA strands are not repulsed, preventing H bond formation between nitrogenous base pairs) The + charged Na+ ions will partially shield the negative charges of the phosphates in the backbone. 2) The temperature of the sample must be 20-25C below the Tm. At this temperature range, random intrastrand H bonding is disrupted, favoring interstrand H bonding between complementary base pairs....
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