Restriction Endonucleases

Restriction Endonucleases - 5-G-A-A-T-T-C-3 3-C-T-T-A-A-G-5...

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Restriction Endonucleases Some endonucleases recognize specific base sequences between 4 and 8 base pairs in length. This class of endonuclease is very critical to the technique of genetic engineering. Type II Restriction Endonucleases recognize a palindromic sequence (reads the same forward as backwards) and make staggered cuts. For example, the EcoRI endonuclease recognizes the palindromic sequence:
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Unformatted text preview: 5'------G-A-A-T-T-C-----3' 3'------C-T-T-A-A-G-----5' Note that reading this sequence on either the top or bottom strands from the 5' to 3' ends it reads in the same order (palindromic). The enzyme will cut the phosphodiester bond between G-A on both strands, resulting in a staggered double-stranded cut of the DNA. 5'------G A-A-T-T-C-----3' 3'------C-T-T-A-A G-----5'...
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.

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