Restriction Enzymes and Genetic Engineering

Restriction Enzymes and Genetic Engineering - 3'--CTTAA...

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Restriction Enzymes and Genetic Engineering This is useful in genetic engineering because the overhangs can be hybridized to like cut DNA from another source and a fragment inserted at the original restriction enzyme site: 5'--G AATTC--insert-G AATTC--3'
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Unformatted text preview: 3'--CTTAA G--insert-CTTAA G--5' | V 5'--G A-A-T-T-C---insert-G A-A-T-T-C--3' 3'--C-T-T-A-A G---insert-C-T-T-A-A G--5' Enzymes called ligases can reform the phosphodiester bonds -: 5'--G-A-A-T-T-C---insert-G-A-A-T-T-C--3' 3'--C-T-T-A-A-G---insert-C-T-T-A-A-G--5'...
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.

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