Semi Conservative Replication

Semi Conservative Replication - in the above experiment...

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Semi Conservative Replication Proven by the Meselson-Stahl experiment of 1958 E. coli Bacteria grown in 15N media so that the "heavy" nitrogen becomes incorporated into the nitrogenous bases. Allowed time for one cycle of DNA replication in 14N. DNA extracted and subjected to Cesium chloride - Density Gradient Centrifugation. This technique allows for the separation of molecules based upon their relative densities. 15N labeled DNA shows a lower band position than 14N labeled DNA following centrifugation. DNA extracted from the E. coli
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Unformatted text preview: in the above experiment showed an intermediate band position (intermediate between that of 15N and 14N) which can result only from one strand containing N15 and one strand containing N14. This result proved that replication is semi-conservative and that the new DNA is a hybrid DNA containing 1 parental strand and 1 daughter strand. If replication had been conservative, there would have been one band at the 15N position and a second band at the 14N position. See page 94 for figure describing Meselson-Stahl experiment....
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.

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