The Holliday Model - The Holliday Model 1964 Molecular...

Info icon This preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
The Holliday Model 1964 Molecular model of recombination: Holliday Model Named after Robin Holliday This model has continued to provide the basis for current thinking about recombination mechanisims, although the model has been modified in recent years. Original model: 1) Single-stranded nicks are introduced at the same position on both parental DNA molecules. 2) Each nicked strand then invades the other DNA molecule by complementary base pairing 3) Ligation produces a crossed-strand intermediate called a Holliday junction 4) Once a Holliday junction is formed, it can be resolved by nicking and rejoining of the crossed strands to yield recombinant or nonrecombinantheteroduplexes (Fig. 5.32). There is direct EM evidence for formation of the Holliday junction (Fig. 5.31) Recombinent heteroduplexes : Resulting DNA molecules are a combination of both parental DNA molecules. Nonrecombinent heteroduplexes : Except for heteroduplex region resulting DNA molecules contain only DNA from one parent molecule.
Image of page 1
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern