The integrated plasmid DNA

The integrated plasmid DNA - plasmid replication regulated...

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The integrated plasmid DNA (termed an episome) can be excised from the bacterial chromosome and be re-established in a plasmid form. Adjacent bacterial genes are often excised too. A plasmid the contains some DNA from the bacterial chromosome is termed an F' plasmid. The F' plasmid can be transfered to an F- female bacterium and complete transfer normally occurs. The recipients are converted into F+ males and there is usually stable retention of the F plasmid and the chromosomal genes. Copy number [i.e. low copy #, high copy #] of plasmids in a cell is a characteristic of the plasmid. F plasmids are always present in only 1 or 2 copies per host cell.
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Unformatted text preview: plasmid replication regulated in coordination with replication of bacterial chromosome regulation at the level of initiation of replication Certain plasmids cannot be maintained together in the same cell. This phenomenon is refered to as plasmid incompatability . Incompatible plasmids: use same mechanism to regulate replication initiation. Such two plasmids together will occur in a specific copy number in the cell, but there is no mechanism that ensures their equal number. By chance one plasmid is often lost after a series of cell divisions....
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This document was uploaded on 11/03/2011 for the course BIOLOGY MCB2010 at Broward College.

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