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The integrated plasmid DNA - • plasmid replication...

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The integrated plasmid DNA (termed an episome) can be excised from the bacterial chromosome and be re-established in a plasmid form. Adjacent bacterial genes are often excised too. A plasmid the contains some DNA from the bacterial chromosome is termed an F' plasmid. The F' plasmid can be transfered to an F- female bacterium and complete transfer normally occurs. The recipients are converted into F+ males and there is usually stable retention of the F plasmid and the chromosomal genes. Copy number [i.e. low copy #, high copy #] of plasmids in a cell is a characteristic of the plasmid. F plasmids are always present in only 1 or 2 copies per host cell.
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Unformatted text preview: • plasmid replication regulated in coordination with replication of bacterial chromosome • regulation at the level of initiation of replication Certain plasmids cannot be maintained together in the same cell. This phenomenon is refered to as plasmid incompatability . Incompatible plasmids: use same mechanism to regulate replication initiation. Such two plasmids together will occur in a specific copy number in the cell, but there is no mechanism that ensures their equal number. By chance one plasmid is often lost after a series of cell divisions....
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