Transposase specifically cleaves at the ends of the inverted repeats of the insertion sequence

Transposase specifically cleaves at the ends of the inverted repeats of the insertion sequence

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Transposase specifically cleaves at the ends of the inverted repeats of the insertion sequence ( cleavage of a specific DNA sequence )...... but transposase also cleaves the target sequence . The target sequence is cleaved via staggered cuts. Transposase then joints the overhanging ends of the target sequence to the
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Unformatted text preview: transposable element. The resulting short strech of single-stranded DNA on both sides of the transposon is finally repaired via DNA polymerase and DNA ligase ----- transposition always creates a short direct repeat of the target sequence that flanks the transposon! (This is true for any transposon.)...
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