Transposition via DNA intermediates

Transposition via DNA intermediates -...

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Transposition via DNA intermediates : The maize transposons first discovered by McClintock but also all known bacterial transposons move via DNA intermediates . Simplest transposable element: insertion sequence : consists of: gene encoding the enzyme transposase (which catalyzes transposition) this includes promoter and transcription and translation termination signals. The insertion sequence is always flanked by short inverted repeats . AGTC. .............................................. GACT TCAG.
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Unformatted text preview: ............................................... CTGA Each insertion sequence is given an IS designation followed by a number. Insertion sequences contain from ~800 to 2000 nucleotides. Insertion sequences move by conservative transposition, which means that they are excised from one site and transposed to a second site without replication of the sequence. Mechanism of transposition by insertion sequences: (Fig. 5. 45) catalyzed by the enzyme they code for: transposase....
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